DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39]
Catalog# ET7107-14
DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39]
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WB
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IHC-P
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Human
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Rat
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unconjugated
Overview
Product Name
DNA Ligase I Recombinant Rabbit Monoclonal Antibody [JB43-39]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human DNA Ligase I aa 1-131 / 919.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 102 kDa
Positive Control
Daudi cell lysate, HeLa cell lysate, Jurkat cell lysate, human tonsil tissue, human spleen tissue, rat testis tissue.
Conjugation
unconjugated
Clone Number
JB43-39
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:50-1:200
Target
Function
DNA ligase 1 is an enzyme that in humans is encoded by the LIG1 gene. DNA ligase I is the only known eukaryotic DNA ligase involved in both DNA replication and repair, making it the most studied of the ligases. LIG1 encodes DNA ligase I, which functions in DNA replication and the base excision repair process. Eukaryotic DNA ligase 1 catalyzes a reaction that is chemically universal to all ligases. DNA ligase 1 utilizes adenosine triphosphate (ATP) to catalyze the energetically favorable ligation events in both DNA replication and repair. During the synthesis phase (S-phase) of the eukaryotic cell cycle, DNA replication occurs. DNA ligase 1 is responsible for joining Okazaki fragments formed during discontinuous DNA synthesis on the DNA's lagging strand after DNA polymerase δ has replaced the RNA primer nucleotides with DNA nucleotides. If the Okazaki fragments are not properly ligated together, the unligated DNA (containing a ‘nick’) could easily degrade to a double strand break, a phenomenon known to cause genetic mutations.
Background References
1. Pascal J M et al. Human DNA ligase I completely encircles and partially unwinds nicked DNA. Nature 432:473-478 (2004).
2. Baple E L et al. Hypomorphic PCNA mutation underlies a human DNA repair disorder. J Clin Invest 124:3137-3146 (2014).
Sequence Similarity
Belongs to the ATP-dependent DNA ligase family.
Subcellular Location
Nucleus.
Synonyms
DNA ligase 1 antibody
DNA ligase I antibody
DNLI1_HUMAN antibody
LIG 1 antibody
lig1 antibody
Ligase I DNA ATP dependent antibody
MGC117397 antibody
MGC130025 antibody
Polydeoxyribonucleotide synthase [ATP] 1 antibody
Images
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Western blot analysis of DNA Ligase I on different lysates with Rabbit anti-DNA Ligase I antibody (ET7107-14) at 1/1,000 dilution.
Lane 1: Daudi cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 140 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-DNA Ligase I antibody (ET7107-14) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-14) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-DNA Ligase I antibody (ET7107-14) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-14) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-DNA Ligase I antibody (ET7107-14) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-14) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"