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E2F2 Recombinant Rabbit Monoclonal Antibody [SN201-04]

Usd: 385

Specification

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Catalog# ET1611-88

E2F2 Recombinant Rabbit Monoclonal Antibody [SN201-04]

Application

  • WB

  • FC

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

E2F2 Recombinant Rabbit Monoclonal Antibody [SN201-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human E2F2 aa 283-415 / 437.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, FC

Molecular Weight

48 kDa

Positive Control

K562 cell lysate, 293T cell lysate, HepG2 cell lysate, K562.

Conjugation

unconjugated

Clone Number

SN201-04

RRID

AB_3070068

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

Target

Function

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.

Background References

1. Mei Z et al. FBXO32 Targets c-Myc for Proteasomal Degradation and Inhibits c-Myc Activity. J Biol Chem 290:16202-14 (2015).

2. Perry MC et al. ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction. Mol Cell Biol 34:4232-43 (2014).

Sequence Similarity

Belongs to the E2F/DP family.

Tissue Specificity

Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

Post-translational Modification

Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q14209 Human

SwissProt: P56931 Mouse

Entrez Gene: 684111 Rat

Synonyms

dE2F2 antibody

E2F transcription factor 2 antibody

E2F-2 antibody

E2F2 antibody

E2F2_HUMAN antibody

Transcription factor E2F2 antibody

Images

  • Western blot analysis of E2F2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1611-88" style="font-weight: bold;text-decoration: underline;">ET1611-88</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: K562 cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: HepG2 cell lysate

    Western blot analysis of E2F2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: K562 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: HepG2 cell lysate

  • Flow cytometric analysis of E2F2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1611-88" style="font-weight: bold;text-decoration: underline;">ET1611-88</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of E2F2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-88, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation