ENTPD5 Recombinant Rabbit Monoclonal Antibody [JE47-87]
Catalog# ET7109-70
ENTPD5 Recombinant Rabbit Monoclonal Antibody [JE47-87]
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WB
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IHC-P
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
ENTPD5 Recombinant Rabbit Monoclonal Antibody [JE47-87]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human ENTPD5 aa 80-220 / 428.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 47 kDa
Positive Control
Human kidney tissue lysates, human kidney tissue.
Conjugation
unconjugated
Clone Number
JE47-87
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50
Target
Function
The protein encoded by this gene is similar to E-type nucleotidases (NTPases)/ecto-ATPase/apyrases. NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD5 contains 4 apyrase-conserved regions which is characteristic of NTPases. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
Background References
1. Mulero JJ et al. CD39-L4 is a secreted human apyrase, specific for the hydrolysis of nucleoside diphosphates. J. Biol. Chem. 274:20064-20067 (1999).
Sequence Similarity
Belongs to the GDA1/CD39 NTPase family.
Tissue Specificity
Expressed in adult liver, kidney, prostate, testis and colon. Much weaker expression in other tissues.
Post-translational Modification
N-glycosylated; high-mannose type (By similarity). Glycosylation is not essential for enzymatic activity.
Subcellular Location
Endoplasmic reticulum. Secreted.
Synonyms
CD39 like 4 antibody
AI196558 antibody
AI987697 antibody
CD39 antigen like 4 antibody
CD39 antigen-like 4 antibody
CD39 like 4 antibody
CD39L4 antibody
Ectonucleoside triphosphate diphosphohydrolase 5 antibody
ENTP5_HUMAN antibody
Entpd5 antibody
ExpandImages
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Western blot analysis of ENTPD5 on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ENTPD5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-70, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"