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PDIA3 / ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44]

Usd: 385

Specification

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Catalog# ET1703-68

PDIA3 / ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44]

Application

  • WB

  • IP

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PDIA3 / ERp57 Recombinant Rabbit Monoclonal Antibody [JM11-44]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PDIA3 aa 175-208 / 505.

Species Reactivity

Human

Validated Applications

WB, IP, IHC-P

Molecular Weight

Predicted band size: 57 kDa

Positive Control

HepG2 cell lysate, LNCaP cell lysate, human kidney tissue, human pancreas tissue, human uterus tissue.

Conjugation

unconjugated

Clone Number

JM11-44

RRID

AB_3070423

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

Target

Function

Mammals defend themselves against intracellular pathogens through presentation of cytoplasmically-derived short pathogenic peptides to the cell surface of cytotoxic T lymphocytes, which subsequently leads to cytotoxic events with respect to the affected cell. Antigen presentation is mediated by major histocompatibility complex (MHC) class I molecules, which bind and coordinate short pathogenic peptides. MHC class I molecules assemble in the endoplasmic reticulum with chaperones before binding to the transporter associated with antigen processing (TAP). ERp57, also designated GRP57, GRP58, ERp60 and ERp61, is a component of the MHC class I pathway that appears to interact with MHC class I molecules before they associate with TAP. The human ERp57 gene maps to chromosome 15q15 and encodes a 505 amino acid protein. ERp57 has two Trp-Cys-Gly-His-Cys-Lys motifs completely conserved among the mammals. ERp57 may act as a protease, a protein disulfide isomerase, a phospholipase or a combination of these.

Background References

1. He Y et al. Largescale Transcriptomics Analysis Suggests Over-Expression of BGH3, MMP9 and PDIA3 in Oral Squamous Cell Carcinoma. PLoS One 11:e0146530 (2016).

2. Kodavanti PR et al. Developmental exposure to a commercial PBDE mixture: effects on protein networks in the cerebellum and hippocampus of rats. Environ Health Perspect 123:428-36 (2015).

Sequence Similarity

Belongs to the protein disulfide isomerase family.

Tissue Specificity

Detected in the flagellum and head region of spermatozoa (at protein level).

Subcellular Location

Endoplasmic reticulum lumen. Melanosome.

UNIPROT

SwissProt: P30101 Human

Synonyms

58 kDa glucose regulated protein antibody

58 kDa glucose-regulated protein antibody

58 kDa microsomal protein antibody

Disulfide isomerase ER 60 antibody

Disulfide isomerase ER-60 antibody

Endoplasmic reticulum resident protein 57 antibody

Endoplasmic reticulum resident protein 60 antibody

ER p57 antibody

ER protein 57 antibody

ER protein 60 antibody

Expand

Images

  • Western blot analysis of PDIA3 / ERp57 on different lysates with Rabbit anti-PDIA3 / ERp57 antibody (<a href="/products/ET1703-68" style="font-weight: bold;text-decoration: underline;">ET1703-68</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: LNCaP cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 57 kDa<br /><br />Exposure time: 15 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-68" style="font-weight: bold;text-decoration: underline;">ET1703-68</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PDIA3 / ERp57 on different lysates with Rabbit anti-PDIA3 / ERp57 antibody (ET1703-68) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: LNCaP cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

    Exposure time: 15 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-68) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of PDIA3 / ERp57 on different lysates with Rabbit anti-PDIA3 / ERp57 antibody (<a href="/products/ET1703-68" style="font-weight: bold;text-decoration: underline;">ET1703-68</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-PDIA3 / ERp57 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 21 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1703-68" style="font-weight: bold;text-decoration: underline;">ET1703-68</a>) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of PDIA3 / ERp57 on different lysates with Rabbit anti-PDIA3 / ERp57 antibody (ET1703-68) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-PDIA3 / ERp57 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 50 kDa

    Exposure time: 21 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-68) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-ERp57 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation