Exportin-5 Recombinant Rabbit Monoclonal Antibody [JE40-91]
Catalog# ET7109-18
Exportin-5 Recombinant Rabbit Monoclonal Antibody [JE40-91]
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WB
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IHC-P
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FC
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Exportin-5 Recombinant Rabbit Monoclonal Antibody [JE40-91]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Exportin-5 aa 1-240 / 1,204.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC, IF-Cell, IF-Tissue
Molecular Weight
136 kDa
Positive Control
293, rat epididymis tissue, human lung cancer tissue, human thyroid gland tissue, human breast tissue, mouse testis tissue, K562.
Conjugation
unconjugated
Clone Number
JE40-91
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:100-1:400
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FC
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1:50-1:100
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IF-Cell
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1:100
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IF-Tissue
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1:100
Target
Function
The karyopherin-related nuclear transport factor Exportin 5, also known as Exp5, preferentially recognizes and transports RNAs containing minihelix motifs, structural cis-acting export elements that comprise a double-stranded stem (14 nucleotides) with a base-paired 5' end and a 3-8-nucleotide protruding 3' end. Exportin 5 also mediates protein transport between the nuclear and cytoplasmic compartment. Exportin 5 belongs to a large family of karyopherins and stimulates nuclear export of dsRNA binding proteins eEF1A and tRNA.
Background References
1. Yi R et al. Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs. Genes Dev 17:3011-3016 (2003).
2. Yi R et al. Overexpression of exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs. RNA 11:220-226 (2005).
Sequence Similarity
Belongs to the exportin family.
Tissue Specificity
Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Subcellular Location
Cytoplasm. Nucleus.
UNIPROT
Synonyms
Exp 5 antibody
Exp5 antibody
Exportin 5 antibody
Exportin-5 antibody
Exportin5 antibody
FLJ14239 antibody
FLJ32057 antibody
FLJ45606 antibody
KIAA1291 antibody
OTTHUMP00000016472 antibody
ExpandImages
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Western blot analysis of Exportin-5 on 293 cell using anti-Exportin-5 antibody at 1/2,000 dilution.
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Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"