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FABP3 Recombinant Rabbit Monoclonal Antibody [JU73-03]

Usd: 385

Specification

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Catalog# ET1706-50

FABP3 Recombinant Rabbit Monoclonal Antibody [JU73-03]

Application

  • WB

  • IHC-P

Reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

FABP3 Recombinant Rabbit Monoclonal Antibody [JU73-03]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human FABP3 aa 1-50 / 133.

Species Reactivity

Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

15 kDa

Positive Control

Mouse heart tissue lysates, rat heart tissue, mouse heart tissue.

Conjugation

unconjugated

Clone Number

JU73-03

RRID

AB_3070670

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

Target

Function

Fatty acid-binding proteins, designated FABPs, are a family of homologous cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epidermis (E-FABP, also designated psoriasis-associated FABP or PA-FABP), muscle and heart (H-FABP, also designated mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP), myelin (M-FABP) and testis (T-FABP).

Background References

1. Baati N et al. Myostatin deficiency is associated with lipidomic abnormalities in skeletal muscles. Biochim Biophys Acta 1862:1044-1055 (2017).

2. Del Collado M et al. Fatty Acid Binding Protein 3 And Transzonal Projections Are Involved In Lipid Accumulation During In Vitro Maturation Of Bovine Oocytes. Sci Rep 7:264

Sequence Similarity

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P11404 Mouse

SwissProt: P07483 Rat

Synonyms

422 protein antibody

Cardiac Fatty Acid Binding Protein antibody

FABP 11 antibody

FABP 3 antibody

FABP11 antibody

FABP3 antibody

FABPH_HUMAN antibody

Fatty acid binding protein 11 antibody

Fatty acid binding protein 3 antibody

Fatty acid binding protein 3 muscle and heart antibody

Expand

Images

  • Western blot analysis of FABP3 on mouse heart tissue lysates with Rabbit anti-FABP3 antibody (<a href="/products/ET1706-50" style="font-weight: bold;text-decoration: underline;">ET1706-50</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 15 kDa<br />Observed band size: 15 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-50" style="font-weight: bold;text-decoration: underline;">ET1706-50</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FABP3 on mouse heart tissue lysates with Rabbit anti-FABP3 antibody (ET1706-50) at 1/500 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-50) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-FABP3 antibody (<a href="/products/ET1706-50" style="font-weight: bold;text-decoration: underline;">ET1706-50</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-50" style="font-weight: bold;text-decoration: underline;">ET1706-50</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-FABP3 antibody (ET1706-50) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-50) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-FABP3 antibody (<a href="/products/ET1706-50" style="font-weight: bold;text-decoration: underline;">ET1706-50</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-50" style="font-weight: bold;text-decoration: underline;">ET1706-50</a>) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-FABP3 antibody (ET1706-50) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-50) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"