Fas / CD95 Recombinant Rabbit Monoclonal Antibody [JJ0942]
Catalog# ET1701-92
Fas / CD95 Recombinant Rabbit Monoclonal Antibody [JJ0942]
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WB
-
IF-Tissue
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IHC-P
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Human
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unconjugated
Overview
Product Name
Fas / CD95 Recombinant Rabbit Monoclonal Antibody [JJ0942]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Fas aa 130-335 / 335.
Species Reactivity
Human
Validated Applications
WB, IF-Tissue, IHC-P
Molecular Weight
Predicted band size: 37 kDa
Positive Control
A431 cell lysate, Hela cell lysate, human tonsil tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
JJ0942
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:500-1:2,000
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IF-Tissue
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1:50-1:200
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IHC-P
-
1:50-1:200
Target
Function
The Fas receptor, also known as Fas, FasR, apoptosis antigen 1 (APO-1 or APT), cluster of differentiation 95 (CD95) or tumor necrosis factor receptor superfamily member 6 (TNFRSF6), is a protein that in humans is encoded by the FAS gene.Fas was first identified using a monoclonal antibody generated by immunizing mice with the FS-7 cell line. Thus, the name Fas is derived from FS-7-associated surface antigen.The Fas receptor is a death receptor on the surface of cells that leads to programmed cell death (apoptosis) if it binds its ligand, Fas ligand (FasL). It is one of two apoptosis pathways, the other being the mitochondrial pathway.[8]
Background References
1. Wang J et al. Mechanism of QSYQ on anti-apoptosis mediated by different subtypes of cyclooxygenase in AMI induced heart failure rats. BMC Complement Altern Med 15:352 (2015).
2. Noyori O et al. Suppression of Fas-mediated apoptosis via steric shielding by filovirus glycoproteins. Biochem Biophys Res Commun 441:994-8 (2013).
Tissue Specificity
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Post-translational Modification
Palmitoylated. Palmitoylation by ZDHHC7 prevents the lysosomal degradation of FAS regulating its expression at the plasma membrane.; N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
Subcellular Location
Cell membrane, Secreted.
UNIPROT
Synonyms
ALPS 1A antibody
ALPS1A antibody
APO 1 antibody
Apo 1 antigen antibody
APO 1 cell surface antigen antibody
Apo-1 antigen antibody
APO1 antibody
Apo1 antigen antibody
APO1 cell surface antigen antibody
Apoptosis antigen 1 antibody
ExpandImages
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Western blot analysis of Fas / CD95 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: Hela cell lysate -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Fas / CD95 antibody (ET1701-92) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-92) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Fas / CD95 antibody (ET1701-92) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-92) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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