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GAB1 Recombinant Rabbit Monoclonal Antibody [JU47-19]

Usd: 385

Specification

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Catalog# ET7106-70

GAB1 Recombinant Rabbit Monoclonal Antibody [JU47-19]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GAB1 Recombinant Rabbit Monoclonal Antibody [JU47-19]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human GAB1 aa 450-500 .

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Molecular Weight

77 kDa

Positive Control

Human small intestine tissue lysate, LOVO, MCF-7, SH-SY5Y, human skin tissue, rat epididymis tissue, Hela.

Conjugation

unconjugated

Clone Number

JU47-19

RRID

AB_3070690

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Growth factor triggering of protein tyrosine kinase receptors induces signals that cascade to the nucleus, activating mitogenic as well as other responses. Critical components of this process include adapter proteins such as Shc, IRS-1 and Gab 1 (GRB-associated binder-1) that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate. Shc and Gab 1 also participate in insulin signaling by linking the insulin receptor to Ras by forming complexes with GRB2 (another adapter protein) and Sos independently of IRS-1. Gab 1 is also thought to be involved in the EGF receptor signaling pathway.

Background References

1. Podar K et al. Critical role for hematopoietic cell kinase (Hck)-mediated phosphorylation of Gab1 and Gab2 docking proteins in interleukin 6-induced proliferation and survival of multiple myeloma cells. J Biol Chem 279:21658-21665 (2004).

2. Brdicka T et al. Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling. J Exp Med 196:1617-1626 (2002).

Sequence Similarity

Belongs to the GAB family.

Post-translational Modification

Phosphorylated in response to FGFR1 activation. Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains. Phosphorylated on tyrosine residues by HCK upon IL6 signaling.

Subcellular Location

Cytosol.

UNIPROT

SwissProt: Q13480 Human

Synonyms

Gab 1 antibody

GAB1 antibody

GAB1_HUMAN antibody

GRB 2 associated binder 1 antibody

GRB 2 associated binding protein 1 antibody

GRB2 associated binding protein 1 isoform a antibody

GRB2 associated binding protein 1 isoform b antibody

GRB2-associated binder 1 antibody

GRB2-associated-binding protein 1 antibody

Growth factor receptor bound protein 2-associated protein 1 antibody

Images

  • Western blot analysis of GAB1 on human small intestine tissue lysate using anti-GAB1 antibody at 1/500 dilution.

    Western blot analysis of GAB1 on human small intestine tissue lysate using anti-GAB1 antibody at 1/500 dilution.

  • ICC staining GAB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining GAB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining GAB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining GAB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining GAB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining GAB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-GAB1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-GAB1 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GAB1 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GAB1 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation