Galectin 8 Recombinant Rabbit Monoclonal Antibody [JB85-35]
Catalog# ET7107-56
Galectin 8 Recombinant Rabbit Monoclonal Antibody [JB85-35]
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WB
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Galectin 8 Recombinant Rabbit Monoclonal Antibody [JB85-35]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Galectin 8 aa 1-50 / 317.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Tissue, IHC-P, IP
Target Molecular Weight
Predicted band size: 36 kDa
Positive Control
PANC-1 cell lysate,Mouse testis tissue lysate, rat testis tissue lysate, A431, HepG2, human kidney tissue, human liver tissue, human colon carcinoma tissue, PC-3M.
Conjugation
unconjugated
Clone Number
JB85-35
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Tissue
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1:400-1:800
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IHC-P
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1:50-1:200
Target
Function
Galectin-8 is a protein of the galectin family that in humans is encoded by the LGALS8 gene. This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. Galectin-8, interacts with the mTOR regulatory system composed of SLC38A9, Ragulator, RagAB, RagCD. Galectin-8 controls mTOR causing its inactivation and dissociation from damaged lysosomes, hence transducing the breach of the lysosomal membrane to mTOR. The physiological consequences of mTOR inhibition following lysosomal membrane damage[8] encompass autophagy and metabolic switching.
Background References
1. Thurston T L et al. Galectin 8 targets damaged vesicles for autophagy to defend cells against bacterial invasion. Nature 482:414-418 (2012).
2. Staring J et al. PLA2G16 represents a switch between entry and clearance of Picornaviridae. Nature 541:412-416 (2017).
Tissue Specificity
Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
Subcellular Location
Cytosol.
Synonyms
Gal 8 antibody
Gal-8 antibody
Gal8 antibody
Galectin-8 antibody
galectin-8g antibody
Lectin galactoside binding soluble 8 antibody
LEG8_HUMAN antibody
LGAL S8 antibody
Lgals8 antibody
PCTA 1 antibody
ExpandImages
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Western blot analysis of Galectin 8 on different lysates with Rabbit anti-Galectin 8 antibody (ET7107-56) at 1/5,000 dilution.
Lane 1: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Rat testis tissue lysate
Cell lysates at 10 µg/Lane,tissue lysates at 10 µg/Lane
Exposure time: 62 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7107-56, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 36 kDa
Observed band size: 42 kDa -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Galectin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Galectin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Galectin 8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"