Growth Hormone Recombinant Rabbit Monoclonal Antibody [JM108-09]
Catalog# ET1703-47
Growth Hormone Recombinant Rabbit Monoclonal Antibody [JM108-09]
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WB
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IHC-P
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IP
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Human
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unconjugated
Overview
Product Name
Growth Hormone Recombinant Rabbit Monoclonal Antibody [JM108-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Growth Hormone aa 72-106 / 217.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 25 kDa
Positive Control
Human placenta tissue lysates, human tonsil tissue, human liver carcinoma tissue, human breast carcinoma tissue, human placenta tissue.
Conjugation
unconjugated
Clone Number
JM108-09
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:50-1:1,000
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IP
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Use at an assay dependent concentration.
Target
Function
Pituitary growth hormone (GH), also designated somatotropin, plays a crucial role in stimulating and controlling the growth, metabolism and differentiation of many mammalian cell types by modulating the synthesis of multiple mRNA species. These effects are mediated by the binding of GH to its membrane-bound receptor, GHR, and involve a phosphorylation cascade that results in the modulation of numerous signaling pathways. GH is secreted in a pulsatile pattern which is tightly controlled by the interplay of GH-releasing hormone (GHRH) and somatostatin (SRIF). GHRH and SRIF are the primary hypothalamic factors that determine GH secretion from the somatotroph and regulate GH synthesis and secretory reserve. GH output is also highly sensitive to feedback control by GH itself, as well as by insulin-like growth factor I. GH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone contains 191 amino acid residues with two disulfide bridges.
Background References
1. Kang Y et al. Tim29 is a novel subunit of the human TIM22 translocase and is involved in complex assembly and stability. Elife 5:N/A (2016).
2. Donti TR et al. Expanding the phenotypic spectrum of Succinyl-CoA ligase deficiency through functional validation of a new SUCLG1 variant. Mol Genet Metab 119:68-74 (2016).
Sequence Similarity
Belongs to the somatotropin/prolactin family.
Subcellular Location
Secreted.
UNIPROT
Synonyms
gH antibody
GH-N antibody
GH1 antibody
GHB5 antibody
GHN antibody
Growth hormone 1 antibody
Growth hormone antibody
Growth hormone B5 antibody
Growth hormone, normal antibody
Growth hormone, pituitary antibody
ExpandImages
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Western blot analysis of Growth Hormone on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST in PBS for 1 hour at room temperature. The primary antibody (ET1703-47, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 25 kDa
Observed band size: 25 kDa -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Growth Hormone antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Growth Hormone antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Growth Hormone antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-47, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Growth Hormone antibody (ET1703-47) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-47) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human Growth Hormone Recombinant Rabbit Monoclonal Antibody [PSH12-82] - BSA and Azide free (Detector)
Application: ELISA(Det)
Reactivity: Human
Conjugate: unconjugated
Human Growth Hormone Recombinant Rabbit Monoclonal Antibody [PSH12-81] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human
Conjugate: unconjugated
