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HDAC6 Recombinant Rabbit Monoclonal Antibody [JJ09-09]

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Specification

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Catalog# ET1701-66

HDAC6 Recombinant Rabbit Monoclonal Antibody [JJ09-09]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

  • Green monkey

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HDAC6 Recombinant Rabbit Monoclonal Antibody [JJ09-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human HDAC6 aa 1-50 / 1,215.

Species Reactivity

Human, Green monkey

Validated Applications

WB, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 131 kDa

Positive Control

HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, COS-1 cell lysate, HCT 116 cell lysate, human kidney tissue, human breast tissue.

Conjugation

unconjugated

Clone Number

JJ09-09

RRID

AB_3070230

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:400

  • IP

  • Use at an assay dependent concentration.

Target

Function

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases.

Background References

1. Sánchez de Diego A et al. Dido3-dependent HDAC6 targeting controls cilium size. Nat Commun 5:3500 (2014).

2. Wei LH et al. Histone deacetylase 6 regulates estrogen receptor alpha in uterine leiomyoma. Reprod Sci 18:755-62 (2011).

Sequence Similarity

Belongs to the histone deacetylase family. HD type 2 subfamily.

Post-translational Modification

Phosphorylated by AURKA.; Ubiquitinated. Its polyubiquitination however does not lead to its degradation.; Sumoylated in vitro.

Subcellular Location

Cell projection, Cytoplasm, Cytoskeleton, Nucleus.

UNIPROT

SwissProt: Q9UBN7 Human

Synonyms

CPBHM antibody

FLJ16239 antibody

HD 6 antibody

HD6 antibody

HDAC 6 antibody

HDAC6 antibody

HDAC6_HUMAN antibody

Histone deacetylase 6 (HD6) antibody

Histone deacetylase 6 antibody

JM 21 antibody

Expand

Images

  • Western blot analysis of HDAC6 on different lysates with Rabbit anti-HDAC6 antibody (<a href="/products/ET1701-66" style="font-weight: bold;text-decoration: underline;">ET1701-66</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: COS-1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 131 kDa<br />Observed band size: 160 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1701-66" style="font-weight: bold;text-decoration: underline;">ET1701-66</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HDAC6 on different lysates with Rabbit anti-HDAC6 antibody (ET1701-66) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: COS-1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 131 kDa
    Observed band size: 160 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of HDAC6 on different lysates with Rabbit anti-HDAC6 antibody (<a href="/products/ET1701-66" style="font-weight: bold;text-decoration: underline;">ET1701-66</a>) at 1/1,000 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si HDAC6 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 131 kDa<br />Observed band size: 160 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1701-66" style="font-weight: bold;text-decoration: underline;">ET1701-66</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of HDAC6 on different lysates with Rabbit anti-HDAC6 antibody (ET1701-66) at 1/1,000 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si HDAC6 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 131 kDa
    Observed band size: 160 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HDAC6 antibody (<a href="/products/ET1701-66" style="font-weight: bold;text-decoration: underline;">ET1701-66</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1701-66" style="font-weight: bold;text-decoration: underline;">ET1701-66</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HDAC6 antibody (ET1701-66) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-66) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-HDAC6 antibody (<a href="/products/ET1701-66" style="font-weight: bold;text-decoration: underline;">ET1701-66</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1701-66" style="font-weight: bold;text-decoration: underline;">ET1701-66</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-HDAC6 antibody (ET1701-66) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-66) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation