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HMGCR Recombinant Rabbit Monoclonal Antibody [JF0981]

Usd: 385

Specification

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Catalog# ET1702-41

HMGCR Recombinant Rabbit Monoclonal Antibody [JF0981]

Application

  • WB

  • IP

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HMGCR Recombinant Rabbit Monoclonal Antibody [JF0981]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human HMGCR aa 438-489 / 888.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP, IHC-P

Molecular Weight

Predicted band size: 97 kDa

Positive Control

HeLa cell lysate, THP-1 cell lysate, HepG2 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human small intestine tissue.

Conjugation

unconjugated

Clone Number

JF0981

RRID

AB_3070303

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • Use at an assay dependent concentration.

  • IHC-P

  • 1:100

Target

Function

The human enzyme hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) limits the rate of cholesterol synthesis, a necessary process for cellular growth, in liver tissue. Phosphorylation of HMGCR inactivates the enzyme, which occurs via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from the product of the reductase reaction. Inhibitors of HMGCR (statins) exert anti-inflammatory effects and decrease the frequency of cardiovascular events by lowering plasma cholesterol. Additionally, intermediate products along the pathway catalyzed by HMGCR, which modulate signal transducing proteins such as Ras, provide possible ties between HMGCR regulation and new chemotherapeutic methods.

Background References

1. Hu HJ et al. Ethanol extract of Zhongtian hawthorn lowers serum cholesterol in mice by inhibiting transcription of 3-hydroxy-3-methylglutaryl-CoA reductase via nuclear factor-kappa B signal pathway. Exp Biol Med (Maywood) 241:667-74 (2016).

2. Rolyan H et al. Defects of Lipid Synthesis Are Linked to the Age-Dependent Demyelination Caused by Lamin B1 Overexpression. J Neurosci 35:12002-17 (2015).

Sequence Similarity

Belongs to the HMG-CoA reductase family.

Post-translational Modification

N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner.; Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1. This INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78 or RNF145, initiating ubiquitination of the reductase. The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97. Lys-248 is the main site of ubiquitination. Ubiquitination is enhanced by the presence of a geranylgeranylated protein.

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: P04035 Human

SwissProt: Q01237 Mouse

SwissProt: P51639 Rat

Synonyms

3 hydroxy 3 methylglutaryl CoA reductase antibody

3 hydroxy 3 methylglutaryl Coenzyme A reductase antibody

3 hydroxymethylglutaryl CoA reductase antibody

3-hydroxy-3-methylglutaryl CoA reductase (NADPH) antibody

3-hydroxy-3-methylglutaryl-coenzyme A reductase antibody

3H3M antibody

HMDH_HUMAN antibody

HMG CoA reductase antibody

HMG CoAR antibody

HMG-CoA reductase antibody

Expand

Images

  • Western blot analysis of HMGCR on different lysates with Rabbit anti-HMGCR antibody (<a href="/products/ET1702-41" style="font-weight: bold;text-decoration: underline;">ET1702-41</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: THP-1 cell lysate<br />Lane 3: HepG2 cell lysate<br />Lane 4: Mouse testis tissue lysate<br />Lane 5: Rat testis tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 98 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 1 minute 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-41" style="font-weight: bold;text-decoration: underline;">ET1702-41</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HMGCR on different lysates with Rabbit anti-HMGCR antibody (ET1702-41) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: THP-1 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: Mouse testis tissue lysate
    Lane 5: Rat testis tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 98 kDa
    Observed band size: 100 kDa

    Exposure time: 1 minute 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-41) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • HMGCR was immunoprecipitated in 0.2mg HeLa cell lysate with <a href="/products/ET1702-41" style="font-weight: bold;text-decoration: underline;">ET1702-41</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/ET1702-41" style="font-weight: bold;text-decoration: underline;">ET1702-41</a> at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: HeLa cell lysate (input)<br />Lane 2: Rabbit IgG instead of <a href="/products/ET1702-41" style="font-weight: bold;text-decoration: underline;">ET1702-41</a> in HeLa cell lysate<br />Lane 3: <a href="/products/ET1702-41" style="font-weight: bold;text-decoration: underline;">ET1702-41</a> IP in HeLa cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 43 seconds

    HMGCR was immunoprecipitated in 0.2mg HeLa cell lysate with ET1702-41 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1702-41 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: HeLa cell lysate (input)
    Lane 2: Rabbit IgG instead of ET1702-41 in HeLa cell lysate
    Lane 3: ET1702-41 IP in HeLa cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 43 seconds

  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HMGCR antibody (<a href="/products/ET1702-41" style="font-weight: bold;text-decoration: underline;">ET1702-41</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-41" style="font-weight: bold;text-decoration: underline;">ET1702-41</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HMGCR antibody (ET1702-41) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-41) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

HMGCR Recombinant Rabbit Monoclonal Antibody [JF0981] - BSA and Azide free

Application: WB,IP,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated