IKB beta Recombinant Rabbit Monoclonal Antibody [SN0648]
Catalog# ET1611-1
IKB beta Recombinant Rabbit Monoclonal Antibody [SN0648]
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WB
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IF-Cell
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IHC-P
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IP
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FC
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Human
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Green monkey
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unconjugated
Overview
Product Name
IKB beta Recombinant Rabbit Monoclonal Antibody [SN0648]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human IKB beta aa 322-356 / 356.
Species Reactivity
Human, Green monkey
Validated Applications
WB, IF-Cell, IHC-P, IP, FC
Molecular Weight
Predicted band size: 38 kDa
Positive Control
HeLa cell lysate, Raji cell lysate, Jurkat cell lysate, COS-1 cell lysate, A549, MCF-7, human kidney tissue, HeLa.
Conjugation
unconjugated
Clone Number
SN0648
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
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FC
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1:1,000
Target
Function
NF-kappa-B inhibitor beta is a protein that in humans is encoded by the NFKBIB gene. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
Background References
1. Stewart ME & Roy P Role of cellular caspases, nuclear factor-kappa B and interferon regulatory factors in Bluetongue virus infection and cell fate. Virol J 7:362 (2010).
2. De Barros S et al. Inhibition of human preadipocyte proteasomal activity by HIV protease inhibitors or specific inhibitor lactacystin leads to a defect in adipogenesis, which involves matrix metalloproteinase-9. J Pharmacol Exp Ther 320:291-9 (2007).
Sequence Similarity
Belongs to the NF-kappa-B inhibitor family.
Tissue Specificity
Expressed in all tissues examined.
Post-translational Modification
Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
Subcellular Location
Nucleus, Cytoplasm.
UNIPROT
Synonyms
I kappa B beta antibody
I-kappa-B-beta antibody
IkappaBbeta antibody
IKB beta antibody
IkB-B antibody
IkB-beta antibody
IKBB antibody
IKBB_HUMAN antibody
IkBbeta antibody
NF kappa BIB antibody
ExpandImages
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Western blot analysis of IKB beta on different lysates with Rabbit anti-IKB beta antibody (ET1611-1) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Raji cell lysate
Lane 3: Jurkat cell lysate
Lane 4: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 48 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-1) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining of IKB beta in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of IKB beta in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-1, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IKB beta antibody (ET1611-1) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-1) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of HeLa cells labeling IKB beta.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1611-1, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"