IRAKM Recombinant Rabbit Monoclonal Antibody [SN64-07]
Catalog# ET1611-11
IRAKM Recombinant Rabbit Monoclonal Antibody [SN64-07]
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WB
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IP
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Human
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unconjugated
Overview
Product Name
IRAKM Recombinant Rabbit Monoclonal Antibody [SN64-07]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human IRAKM aa 547-596 / 596.
Species Reactivity
Human
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 68 kDa
Positive Control
Human placenta tissue lysate, human thyroid tissue lysate.
Conjugation
unconjugated
Clone Number
SN64-07
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IP
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Use at an assay dependent concentration.
Target
Function
The interleukin-1 receptor (IL-1R) associated kinase (IRAK) family plays a crucial role in the protective response to pathogens introduced into the human body by inducing acute inflammation followed by additional adaptive immune responses. IRAKs are essential components of the Interleukin-1 receptor signaling pathway and some Toll-like receptor signaling pathways. Toll-like receptors (TLRs) detect microorganisms by recognizing specific pathogen-associated molecular patterns (PAMPs) and IL-1R family members respond the interleukin-1 (IL-1) family cytokines. These receptors initiate an intracellular signaling cascade through adaptor proteins, primarily, MyD88. This is followed by the activation of IRAKs. TLRs and IL-1R members have a highly conserved amino acid sequence in their cytoplasmic domain called the Toll/Interleukin-1 (TIR) domain. The elicitation of different TLRs/IL-1Rs results in similar signaling cascades due to their homologous TIR motif leading to the activation of mitogen-activated protein kinases (MAPKs) and the IκB kinase (IKK) complex, which initiates a nuclear factor-κB (NF-κB) and AP-1-dependent transcriptional response of pro-inflammatory genes. Understanding the key players and their roles in the TLR/IL-1R pathway is important because the presence of mutations causing the abnormal regulation of Toll/IL-1R signaling leading to a variety of acute inflammatory and autoimmune diseases.
Background References
1. Hayashi T et al. Induction of Tolerogenic Dendritic Cells by a PEGylated TLR7 Ligand for Treatment of Type 1 Diabetes. PLoS One 10:e0129867 (2015).
2. Ballinger MN et al. TLR signaling prevents hyperoxia-induced lung injury by protecting the alveolar epithelium from oxidant-mediated death. J Immunol 189:356-64 (2012).
Sequence Similarity
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Tissue Specificity
Expressed predominantly in peripheral blood lymphocytes.
Subcellular Location
Cytoplasm, Nucleus.
UNIPROT
Synonyms
ASRT5 antibody
IL 1 receptor associated kinase M antibody
IL-1 receptor-associated kinase M antibody
IL1 receptor associated kinase M antibody
Interleukin 1 receptor associated kinase 3 antibody
Interleukin 1 receptor associated kinase M antibody
Interleukin-1 receptor-associated kinase 3 antibody
IRAK M antibody
IRAK-3 antibody
IRAK-M antibody
ExpandImages
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Western blot analysis of IRAKM on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human placenta tissue lysate
Lane 2: Human thyroid tissue lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"