Insulin Receptor Beta Recombinant Rabbit Monoclonal Antibody [SN20-13]
Overview
Product Name
Insulin Receptor Beta Recombinant Rabbit Monoclonal Antibody [SN20-13]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Insulin receptor aa 930-970.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P
Molecular Weight
Predicted band size: 156 kDa
Positive Control
HCT 116 cell lysate, MDA-MB-231 cell lysate, HepG2 cell lysate, T-47D cell lysate, Rat liver tissue lysate, mouse skeletal muscle tissue lysates, HepG2, LO2, RH-35, rat liver tissue, mouse liver tissue, human liver tissue.
Conjugation
unconjugated
Clone Number
SN20-13
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
IF-Cell
-
1:100-1:500
-
IF-Tissue
-
1:100-1:500
-
IHC-P
-
1:1,000
Target
Function
The insulin receptor (IR) is a heterodimeric protein complex that has an intracellular β subunit and an extracellular α subunit, which is disulfide- linked to a transmembrane segment. The insulin ligand binds to the IR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to IR induces phosphorylation of intracellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. The human insulin receptor gene maps to chromosome 19p13.3-p13.2 and encodes a 1382 amino acid protein that cleaves to form α and β subunits. Type 1 diabetes is an auto-immune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells. Type 2 diabetes is a condition where cells become resistant to insulin action.
Background References
1. Cao, L. et al. 2012. Hepatic insulin signaling changes: possible mechanism in prenatal hypoxia-increased susceptibility of fatty liver in adulthood. Endocrinology. 153: 4955-65.
2. Ruttenstock, E. et al. 2011. Prenatal administration of retinoic acid upregulates insulin-like growth factor receptors in the nitrofen-induced hypoplastic lung. Birth Defects Res. B Dev. Reprod. Toxicol.. 92: 148-151.
Sequence Similarity
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Tissue Specificity
Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas.
Post-translational Modification
After being transported from the endoplasmic reticulum to the Golgi apparatus, the single glycosylated precursor is further glycosylated and then cleaved, followed by its transport to the plasma membrane.; Autophosphorylated on tyrosine residues in response to insulin. Phosphorylation of Tyr-999 is required for binding to IRS1, SHC1 and STAT5B. Dephosphorylated by PTPRE at Tyr-999, Tyr-1185, Tyr-1189 and Tyr-1190. Dephosphorylated by PTPRF and PTPN1. Dephosphorylated by PTPN2; down-regulates insulin-induced signaling.
Subcellular Location
Cell membrane.
Synonyms
CD220 antibody
HHF5 antibody
HIR A antibody
INSR antibody
Insulin receptor antibody
Insulin receptor subunit alpha antibody
IR antibody
Images
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☑ Knockdown (KD)
Western blot analysis of Insulin Receptor Beta on different lysates with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Insulin Receptor Beta cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 156 kDa
Observed band size: 95 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-90) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Western blot analysis of Insulin Receptor Beta on different lysates with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution.
Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: T-47D cell lysate (low expression) (20 µg/Lane)
Lane 4: Hepa1-6 cell lysate (20 µg/Lane)
Lane 5: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 156 kDa
Observed band size: 95/45 kDa
Exposure time: Lane 1-3: 1 minute 2 seconds; Lane 4-5: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-90) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Insulin Receptor Beta on mouse skeletal muscle tissue lysates with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 156 kDa
Observed band size: 95 kDa
Exposure time: 3 minutes;
4~20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-90) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
ICC staining of Insulin Receptor Beta in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
-
ICC staining of Insulin Receptor Beta in LO2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
-
ICC staining of Insulin Receptor Beta in RH-35 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1611-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
-
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Insulin Receptor Beta antibody (ET1611-90) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-90) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Bifidobacterium longum subsp. longum B-53 Alleviated Abnormalities of Glycolipid Metabolism and Oxidative Stress in T2DM Mice Through Regulating Gut Microbiota and GLP-1 Secretion
Journal: Journal Of Agricultural And Food Chemistry
DOI: 10.1021/acs.jafc.5c07346
IF: 6.2
Application: WB
Reactivity: Mouse
Publish date: 2025 Sept
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