Involucrin Recombinant Rabbit Monoclonal Antibody [JU30-27]
Catalog# ET1706-32
Involucrin Recombinant Rabbit Monoclonal Antibody [JU30-27]
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WB
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Involucrin Recombinant Rabbit Monoclonal Antibody [JU30-27]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Involucrin aa 131-180 / 585.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Molecular Weight
120 kDa
Positive Control
Human skin tissue lysates, human lung carcinoma tissue, A431.
Conjugation
unconjugated
Clone Number
JU30-27
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:200
Target
Function
Involucrin is a precursor protein of the keratinocyte cornified envelope, which is formed beneath the inner surface of the cell membrane during terminal differentiation. During keratinocyte terminal differentiation, glutamine residues of involucrin become covalently cross-linked to other envelope precursors via covalent epsilon-(gamma-glutamyl) lysine bonds. Moreover, its large size allows involucrin to cross-link molecules that are separated by substantial distances in the cornified envelope. These properties allow a single involucrin molecule to form multiple cross-links, in multiple spatial planes, with other envelope precursors.
Background References
1. Chen JQ et al. Regulation of involucrin and signalling pathway in scleroderma epidermal keratinocytes. Postepy Dermatol Alergol. 2024 Dec
2. Yang S et al. Dihydromyrcenol Modulates Involucrin Expression through the Akt Signaling Pathway. Int J Mol Sci. 2024 Feb
Sequence Similarity
Belongs to the involucrin family.
Tissue Specificity
Keratinocytes of epidermis and other stratified squamous epithelia.
Post-translational Modification
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
Subcellular Location
Cytoplasm.
Synonyms
INVO_HUMAN antibody
Involucrin antibody
IVL antibody
Images
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Western blot analysis of Involucrin on human skin tissue lysates with Rabbit anti-Involucrin antibody (ET1706-32) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-32) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Involucrin antibody (ET1706-32) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-32) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"