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K63-linkage Specific Ubiquitin Recombinant Rabbit Monoclonal Antibody [JM09-67]

Usd: 385

Specification

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Catalog# ET1703-07

K63-linkage Specific Ubiquitin Recombinant Rabbit Monoclonal Antibody [JM09-67]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

K63-linkage Specific Ubiquitin Recombinant Rabbit Monoclonal Antibody [JM09-67]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human K63-linkage Specific Ubiquitin aa 36-85.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 15/18/26/77 kDa

Positive Control

Hela cell lysates, 293T, N2A, Hela, human liver cancer tissue, human colon cancer tissue, human spleen tissue.

Conjugation

unconjugated

Clone Number

JM09-67

RRID

AB_3070364

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100-1:500

  • IF-Tissue

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

Target

Function

Ubiquitin is a small (8.6 kDa) regulatory protein found in most tissues of eukaryotic organisms. Four genes in the human genome code for ubiquitin: UBB, UBC, UBA52 and RPS27A. The addition of ubiquitin to a substrate protein is called ubiquitylation (or, alternatively, ubiquitination or ubiquitinylation). Ubiquitylation affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitylation involves three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. Only polyubiquitylation on defined lysines, mostly on K48 and K29, is related to degradation by the proteasome (referred to as the "molecular kiss of death"), while other polyubiquitylations (e.g. on K63, K11, K6 and M1) and monoubiquitylations may regulate processes such as endocytic trafficking, inflammation, translation and DNA repair.

Background References

1. Choi YB & Harhaj EW HTLV-1 tax stabilizes MCL-1 via TRAF6-dependent K63-linked polyubiquitination to promote cell survival and transformation. PLoS Pathog 10:e1004458 (2014).

2. Jadhav S et al. RNA-binding Protein Musashi Homologue 1 Regulates Kidney Fibrosis by Translational Inhibition of p21 and Numb mRNA. J Biol Chem 291:14085-94 (2016).

Sequence Similarity

Belongs to the ubiquitin family.

Post-translational Modification

[Ubiquitin]: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy. Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30.; [Ubiquitin]: Mono-ADP-ribosylated at the C-terminus by PARP9, a component of the PPAR9-DTX3L complex. ADP-ribosylation requires processing by E1 and E2 enzymes and prevents ubiquitin conjugation to substrates such as histones.

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P0CG47 Human

SwissProt: P0CG48 Human

SwissProt: P62979 Human

SwissProt: P62987 Human

SwissProt: P0CG49 Mouse

SwissProt: P62991 Mouse

SwissProt: P0CG51 Rat

SwissProt: P62989 Rat

Synonyms

Epididymis secretory protein Li 50 antibody

FLJ25987 antibody

HEL S 50 antibody

MGC8385 antibody

Polyubiquitin B antibody

RPS 27A antibody

RPS27A antibody

UBA 52 antibody

UBA 80 antibody

UBA52 antibody

Expand

Images

  • Western blot analysis of K63-linkage Specific Ubiquitin on Hela cells lysates using anti-K63-linkage Specific Ubiquitin antibody at 1/1,000 dilution.

    Western blot analysis of K63-linkage Specific Ubiquitin on Hela cells lysates using anti-K63-linkage Specific Ubiquitin antibody at 1/1,000 dilution.

  • ICC staining K63-linkage Specific Ubiquitin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining K63-linkage Specific Ubiquitin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining K63-linkage Specific Ubiquitin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining K63-linkage Specific Ubiquitin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining K63-linkage Specific Ubiquitin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining K63-linkage Specific Ubiquitin in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-K63-linkage Specific Ubiquitin antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation