KMT5A / SETD8 / Pr-SET7 Recombinant Rabbit Monoclonal Antibody [JM73-43]
Catalog# ET1705-59
KMT5A / SETD8 / Pr-SET7 Recombinant Rabbit Monoclonal Antibody [JM73-43]
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WB
-
FC
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Human
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Mouse
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unconjugated
Overview
Product Name
KMT5A / SETD8 / Pr-SET7 Recombinant Rabbit Monoclonal Antibody [JM73-43]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human KMT5A aa 51-100 / 393.
Species Reactivity
Human, Mouse
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 43 kDa
Positive Control
Mouse kidney tissue lysates, Mouse liver tissue lysates, 293T cell lysates, 293 cell lysates, 293T cells.
Conjugation
unconjugated
Clone Number
JM73-43
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:50-1:100
Target
Function
Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. All monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions , benef playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contribute to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53 / TP53 at 'Lys-382', leading to repress p53 / TP53-target genes.
Background References
1. Gursoy-Yuzugullu O et al. Epigenetic therapy with inhibitors of histone methylation suppresses DNA damage signaling and increases glioma cell radiosensitivity. Oncotarget 8:24518-24532 (2017).
2. Zhou HR et al. Relationship between epigenetic changes in Wnt antagonists and acute leukemia. Oncol Rep 37:2663-2671 (2017).
Sequence Similarity
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. PR/SET subfamily.
Post-translational Modification
Acetylated at Lys-172; does not change methyltransferase activity. Deacetylated at Lys-172 by SIRT2; does not change methyltransferase activity.; Ubiquitinated and degraded by the DCX(DTL) complex.
Subcellular Location
Nucleus.
Synonyms
H4 K20 HMTase antibody
H4 K20 specific histone methyltransferase antibody
H4-K20-HMTase SETD8 antibody
Histone H4 K20 methyltransferase antibody
Histone lysine N methyltransferase H4 lysine 20 specific antibody
Histone-lysine N-methyltransferase SETD8 antibody
Lysine N-methyltransferase 5A antibody
N-lysine methyltransferase SETD8 antibody
Pr SET 7 antibody
Pr SET7 antibody
ExpandImages
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Western blot analysis of KMT5A on different lysates using anti-KMT5A antibody at 1/1,000 dilution.
Positive control:
Lane 1: Mouse kidney tissue lysates
Lane 2: Mouse liver tissue lysates
Lane 3: 293T cell lysates
Lane 4: 293 cell lysates
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"