MEIS1 Recombinant Rabbit Monoclonal Antibody [JA13-26]
Catalog# ET1704-84
MEIS1 Recombinant Rabbit Monoclonal Antibody [JA13-26]
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WB
-
IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MEIS1 Recombinant Rabbit Monoclonal Antibody [JA13-26]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MEIS1 aa 328-377 / 390.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 43 kDa
Positive Control
HeLa cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JA13-26
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
Target
Function
Acts as a transcriptional regulator of PAX6. Acts as a transcriptional activator of PF4 in complex with PBX1 or PBX2. Required for hematopoiesis, megakaryocyte lineage development and vascular patterning. May function as a cofactor for HOXA7 and HOXA9 in the induction of myeloid leukemias.
Background References
1. Patel AV et al. An ShRNA Screen Identifies MEIS1 as a Driver of Malignant Peripheral Nerve Sheath Tumors. EBioMedicine 9:110-9 (2016).
2. Dupays L et al. Sequential Binding of MEIS1 and NKX2-5 on the Popdc2 Gene: A Mechanism for Spatiotemporal Regulation of Enhancers during Cardiogenesis. Cell Rep 13:183-95 (2015).
Sequence Similarity
Belongs to the TALE/MEIS homeobox family.
Tissue Specificity
Expressed at low level in normal immunohepatopoietic tissues, including the fetal liver. Expressed in a subset of myeloid leukemia cell lines, with the highest expression seen in those with a megakaryocytic-erythroid phenotype. Also expressed at high levels in the cerebellum.
Subcellular Location
Nucleus.
Synonyms
Homeo box protein Meis1 antibody
Homeobox protein Meis1 antibody
Leukemogenic homolog protein antibody
MEIS 1 antibody
Meis homeo box 1 antibody
Meis homeobox 1 antibody
Meis1 antibody
Meis1 mouse homolog antibody
Meis1 myeloid ecotropic viral integration site 1 homolog antibody
Meis1 myeloid ecotropic viral integration site 1 homolog mouse antibody
ExpandImages
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Western blot analysis of MEIS1 on different lysates with Rabbit anti-MEIS1 antibody (ET1704-84) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 43 kDa
Observed band size: 51 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-84) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"