MEK5 Recombinant Rabbit Monoclonal Antibody [SD208-6]
Catalog# ET1612-34
MEK5 Recombinant Rabbit Monoclonal Antibody [SD208-6]
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WB
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IF-Cell
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
MEK5 Recombinant Rabbit Monoclonal Antibody [SD208-6]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human MEK5 aa 381-330 / 448.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IF-Tissue
Molecular Weight
50 kDa
Positive Control
Hela cell lysates, Hela, A431, SW480.
Conjugation
unconjugated
Clone Number
SD208-6
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
Target
Function
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
Background References
1. Jiang, H. et al. 2014. Restoration of MiR-17/20a in Solid Tumor Cells Enhances the Natural Killer Cell Anti-tumor Activity by Targeting Mekk2. Cancer immunology research. 2: 1-11.
2. Roberts, OL. et al. 2010. ERK5 is required for VEGF-mediated survival and tubular morphogenesis of primary human microvascular endothelial cells. J. Cell. Sci.. 123: 3189-3200.
Sequence Similarity
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Tissue Specificity
Expressed in many adult tissues. Abundant in heart and skeletal muscle.
Post-translational Modification
Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.; Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
Subcellular Location
Spindle, Cytosol, nucleus, cytoplasm.
UNIPROT
Synonyms
Dual specificity mitogen activated protein kinase kinase 5 antibody
Dual specificity mitogen-activated protein kinase kinase 5 antibody
EC 2.7.12.2 antibody
HsT17454 antibody
MAP kinase kinase 5 antibody
MAP kinase kinase MEK5b antibody
MAP2K5 antibody
MAPK/ERK kinase 5 antibody
MAPKK 5 antibody
MAPKK5 antibody
ExpandImages
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Western blot analysis of MEK5 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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ICC staining of MEK5 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of MEK5 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of MEK5 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"