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MRPL28 Recombinant Rabbit Monoclonal Antibody [JE55-90]

Usd: 385

Specification

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Catalog# ET7111-30

MRPL28 Recombinant Rabbit Monoclonal Antibody [JE55-90]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

  • Zebrafish

Conjugation

  • unconjugated

Overview

Product Name

MRPL28 Recombinant Rabbit Monoclonal Antibody [JE55-90]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within N-terminal Human MRPL28.

Species Reactivity

Human, Mouse, Rat, Zebrafish

Validated Applications

WB

Molecular Weight

Predicted band size: 30 kDa

Positive Control

HEK-293 cell lysate, SW620 cell lysate, NIH/3T3 cell lysate, 4T1 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, zebrafish tissue lysates.

Conjugation

unconjugated

Clone Number

JE55-90

RRID

AB_3071087

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

Target

Function

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.

Background References

1. Yang Y. et. al. Characterization of TsMRP-L28, a mitochondrial ribosomal protein L28 from the parasitic nematode Trichinella spiralis. Gene. 2013 Jan

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: Q13084 Human

SwissProt: Q9D1B9 Mouse

Entrez Gene: 497876 Rat

Synonyms

39S ribosomal protein L28 antibody

39S ribosomal protein L28 mitochondrial antibody

39S ribosomal protein L28 mitochondrial precursor antibody

HGNC6756 antibody

L28mt antibody

MAAT 1 antibody

MAAT1 antibody

Melanoma antigen p15 antibody

Melanoma associated antigen recognised by cytotoxic T lymphocytes antibody

Melanoma associated antigen recognized by T lymphocytes antibody

Expand

Images

  • Western blot analysis of MRPL28 on different lysates with Rabbit anti-MRPL28 antibody (<a href="/products/ET7111-30" style="font-weight: bold;text-decoration: underline;">ET7111-30</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: SW620 cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: 4T1 cell lysate<br />Lane 5: Mouse kidney tissue lysate<br />Lane 6: Rat kidney tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 30 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-30" style="font-weight: bold;text-decoration: underline;">ET7111-30</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MRPL28 on different lysates with Rabbit anti-MRPL28 antibody (ET7111-30) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: SW620 cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: 4T1 cell lysate
    Lane 5: Mouse kidney tissue lysate
    Lane 6: Rat kidney tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

    Exposure time: 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-30) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of MRPL28 on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-30" style="font-weight: bold;text-decoration: underline;">ET7111-30</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of MRPL28 on zebrafish tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7111-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"