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MelanA Recombinant Rabbit Monoclonal Antibody [SC56-02]

Usd: 385

Specification

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Catalog# ET1610-47

MelanA Recombinant Rabbit Monoclonal Antibody [SC56-02]

Application

  • IHC-P

  • IF-Tissue

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

MelanA Recombinant Rabbit Monoclonal Antibody [SC56-02]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human MelanA aa 2-43 / 118.

Species Reactivity

Human

Validated Applications

IHC-P, IF-Tissue, WB

Molecular Weight

Predicted band size: 13 kDa

Positive Control

Melanoma tissue lysate, B16F1 cell lysate, human melanoma tissue.

Conjugation

unconjugated

Clone Number

SC56-02

RRID

AB_3069932

Reactivity Data

IHC-P IF-Tissue WB
Human
Tested Tested
Tested Tested
Tested Tested
Mouse
Not Recommended
Not Recommended

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200

  • IF-Tissue

  • 1:200

  • WB

  • 1:1,000

Target

Function

Melanoma-associated antigens recognized by cytotoxic T lymphocytes (CTL) have been grouped into three categories: melanocyte differentiation antigens, cancer/testis-specific antigens and mutated or aberrantly expressed antigens. Many of these antigens consist of peptides that are presented to T cells by HLA molecules; they represent potential targets for cancer immunotherapy. Melan-A (also designated MART-1) is a melanocyte differentiation antigen that is specific to melanomas, melanocyte cell lines and retina. Melan-A peptide is recognized by most HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes in patients with melanoma. Antimelanoma cytotoxic T lymphocytes can be generated with a Melan-A peptide, implicating Melan-A as a potential candidate for antigen-specific immunotherapy in melanoma patients.

Background References

1. Ulmer A et al. Quantitative measurement of melanoma spread in sentinel lymph nodes and survival. PLoS Med 11:e1001604 (2014).

2. Dziasko MA et al. Localisation of epithelial cells capable of holoclone formation in vitro and direct interaction with stromal cells in the native human limbal crypt. PLoS One 9:e94283 (2014).

Tissue Specificity

Expression is restricted to melanoma and melanocyte cell lines and retina.

Post-translational Modification

Acylated.

Subcellular Location

Golgi apparatus, trans-Golgi network membrane, Endoplasmic reticulum membrane, Melanosome.

UNIPROT

SwissProt: Q16655 Human

Synonyms

Antigen LB39 AA antibody

Antigen LB39-AA antibody

Antigen SK29 AA antibody

Antigen SK29-AA antibody

MAR1_HUMAN antibody

MART 1 antibody

MART-1 antibody

MART1 antibody

Melan A antibody

Melan A protein antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human melanoma tissue with Rabbit anti-MelanA antibody (<a href="/products/ET1610-47" style="font-weight: bold;text-decoration: underline;">ET1610-47</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-47" style="font-weight: bold;text-decoration: underline;">ET1610-47</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human melanoma tissue with Rabbit anti-MelanA antibody (ET1610-47) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-47) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: malignant melanoma<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: malignant melanoma

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation