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mGluR5 Recombinant Rabbit Monoclonal Antibody [ST51-00]

Usd: 385

Specification

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Catalog# ET1609-36

mGluR5 Recombinant Rabbit Monoclonal Antibody [ST51-00]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

mGluR5 Recombinant Rabbit Monoclonal Antibody [ST51-00]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human mGluR5 aa 1,163-1,212 / 1,212.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 132 kDa

Positive Control

Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

ST51-00

RRID

AB_3069846

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:200-1:1,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

The mGluR proteins (metabotropic Glutamate Receptors) are members of the G protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic Glutamate Receptors). The eight currently known mGluR proteins are mediated by two G proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR1 and mGluR5 are mediated by a G protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight sub-types of mGluR have an activity mediated by a G protein that inhibits adenylate cyclase activity. mGluR-5, which can interact with SIAH-1, RyR-1, RyR-2, ITPR1, Shank 1, Shank 3 and GRASP, acts as a receptor for glutamate. The PPXXf motif of mGluR-5 binds to HOM1, HOM2 and HOM3.

Background References

1. Lee KW et al. Alteration by p11 of mGluR5 localization regulates depression-like behaviors. Mol Psychiatry 20:1546-56 (2015).

2. Rossignol R et al. Visual sensorial impairments in neurodevelopmental disorders: evidence for a retinal phenotype in fragile x syndrome. PLoS One 9:e105996 (2014).

Sequence Similarity

Belongs to the G-protein coupled receptor 3 family.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P41594 Human

SwissProt: Q3UVX5 Mouse

SwissProt: P31424 Rat

Synonyms

Glutamate receptor metabotropic 5 antibody

GPRC1E antibody

Grm5 antibody

GRM5_HUMAN antibody

Metabotropic glutamate receptor 5 antibody

Metabotropic glutamate receptor 5 variant F antibody

Metabotropic glutamate receptor 5 variant G antibody

Metabotropic glutamate receptor 5 variant H antibody

mGlu5 antibody

mGluR5 antibody

Expand

Images

  • Western blot analysis of mGluR5 on different lysates with Rabbit anti-mGluR5 antibody (<a href="/products/ET1609-36" style="font-weight: bold;text-decoration: underline;">ET1609-36</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human brain tissue lysate (RIPA lysis)<br />Lane 2: Mouse brain tissue lysate (no heat)<br />Lane 3: Mouse brain tissue lysate (hot lysis)<br />Lane 4: Rat brain tissue lysate (no heat)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 132 kDa<br />Observed band size: 150 kDa<br /><br />Exposure time: Lane 1: 3 minutes; Lane 2-3: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-36" style="font-weight: bold;text-decoration: underline;">ET1609-36</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of mGluR5 on different lysates with Rabbit anti-mGluR5 antibody (ET1609-36) at 1/1,000 dilution.

    Lane 1: Human brain tissue lysate (RIPA lysis)
    Lane 2: Mouse brain tissue lysate (no heat)
    Lane 3: Mouse brain tissue lysate (hot lysis)
    Lane 4: Rat brain tissue lysate (no heat)

    Notice: no heat means the lysate is not boiled.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 132 kDa
    Observed band size: 150 kDa

    Exposure time: Lane 1: 3 minutes; Lane 2-3: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-mGluR5 antibody (<a href="/products/ET1609-36" style="font-weight: bold;text-decoration: underline;">ET1609-36</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-36" style="font-weight: bold;text-decoration: underline;">ET1609-36</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-mGluR5 antibody (ET1609-36) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-mGluR5 antibody (<a href="/products/ET1609-36" style="font-weight: bold;text-decoration: underline;">ET1609-36</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-36" style="font-weight: bold;text-decoration: underline;">ET1609-36</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-mGluR5 antibody (ET1609-36) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-mGluR5 antibody (<a href="/products/ET1609-36" style="font-weight: bold;text-decoration: underline;">ET1609-36</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-36" style="font-weight: bold;text-decoration: underline;">ET1609-36</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-mGluR5 antibody (ET1609-36) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-36) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation