Mib1 Recombinant Rabbit Monoclonal Antibody [JG69-34]
Catalog# ET7108-43
Mib1 Recombinant Rabbit Monoclonal Antibody [JG69-34]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
Mib1 Recombinant Rabbit Monoclonal Antibody [JG69-34]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Mib1 aa 2-93 / 1006.
Species Reactivity
Human, Mouse
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 110 kDa
Positive Control
SH-SY5Y cell lysate, U-87MG cell lysate, Mouse brain tissue lysate, Mouse testis tissue lysate, HUVEC, SH-SY5Y, SiHa, human appendix tissue, human testis tissue.
Conjugation
unconjugated
Clone Number
JG69-34
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation.
Background References
1. Villumsen B H et al. A new cellular stress response that triggers centriolar satellite reorganization and ciliogenesis. EMBO J 32:3029-3040 (2013).
2. Luxan G et al. Mutations in the NOTCH pathway regulator MIB1 cause left ventricular noncompaction cardiomyopathy. Nat Med 19:193-201 (2013).
Tissue Specificity
Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
Post-translational Modification
Ubiquitinated; possibly via autoubiquitination (By similarity). Ubiquitinated; this modification is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock.
Subcellular Location
Plasma membrane. Cytoplasm. Cytoskeleton.
Synonyms
DAPK-interacting protein 1 antibody
Dip 1 antibody
DIP-1 antibody
Dip1 antibody
E3 ubiquitin protein ligase MIB 1 antibody
E3 ubiquitin protein ligase MIB1 antibody
E3 ubiquitin-protein ligase mib1 antibody
KIAA1323 antibody
LVNC7 antibody
MIB antibody
ExpandImages
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Western blot analysis of Mib1 on different lysates with Rabbit anti-Mib1 antibody (ET7108-43) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: U-87MG cell lysate (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (40 µg/Lane)
Lane 4: Mouse testis tissue lysate (40 µg/Lane)
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-43) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HUVEC cells labeling Mib1 with Rabbit anti-Mib1 antibody (ET7108-43) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Mib1 antibody (ET7108-43) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of SH-SY5Y cells labeling Mib1 with Rabbit anti-Mib1 antibody (ET7108-43) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Mib1 antibody (ET7108-43) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of SiHa cells labeling Mib1 with Rabbit anti-Mib1 antibody (ET7108-43) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Mib1 antibody (ET7108-43) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI. -
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Mib1 antibody (ET7108-43) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-43) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Mib1 antibody (ET7108-43) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-43) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"