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Mitofusin 1 Recombinant Rabbit Monoclonal Antibody [JF0954]

Usd: 385

Specification

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Catalog# ET1702-01

Mitofusin 1 Recombinant Rabbit Monoclonal Antibody [JF0954]

Application

  • WB

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Mitofusin 1 Recombinant Rabbit Monoclonal Antibody [JF0954]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Mitofusin 1 aa 491-620 / 741.

Species Reactivity

Human

Validated Applications

WB, IP

Molecular Weight

Predicted band size: 84 kDa

Positive Control

K-562 cell lysates.

Conjugation

unconjugated

Clone Number

JF0954

RRID

AB_3070266

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2) are homologs for the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins and are mediators of mitochondrial fusion. A GTPase domain is required for Mfn protein function but the molecular mechanisms of the GTPase-dependent reaction as well as the functional division of the two Mfn proteins are unknown. They are essential for embryonic development and may play a role in the pathobiology of obesity. Although the Mfn1 and Mfn2 genes are broadly expressed, they show different levels of expression in different tissues. Two Mfn1 transcripts are elevated in heart, while Mfn2 mRNA is abundantly expressed in heart and muscle tissue but present only at low levels in many other tissues. Mfn1 localizes to mitochondria and participates in at least two different high molecular weight protein complexes in a GTP-dependent manner. Purified recombinant Mfn1 exhibited approximately eightfold higher GTPase activity than Mfn2.

Background References

1. Malhotra A et al. Sonic Hedgehog Signaling Drives Mitochondrial Fragmentation by Suppressing Mitofusins in Cerebellar Granule Neuron Precursors and Medulloblastoma. Mol Cancer Res 14:114-24 (2016).

2. Bruns DR et al. Mitochondrial integrity in a neonatal bovine model of right ventricular dysfunction. Am J Physiol Lung Cell Mol Physiol 308:L158-67 (2015).

Sequence Similarity

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.

Tissue Specificity

Detected in kidney and heart (at protein level). Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.

Post-translational Modification

Ubiquitinated by non-degradative ubiquitin by PRKN. Deubiquitination by USP30 inhibits mitochondrial fusion (By similarity). Ubiquitinated by MARCHF5. When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN.

Subcellular Location

Mitochondrion outer membrane, Cytoplasm.

UNIPROT

SwissProt: Q8IWA4 Human

Synonyms

Fzo homolog antibody

Hfzo1 antibody

Hfzo2 antibody

MFN 1 antibody

Mfn1 antibody

MFN1_HUMAN antibody

Mitochondrial transmembrane GTPase Fzo 1 antibody

Mitochondrial transmembrane GTPase FZO 2 antibody

Mitochondrial transmembrane GTPase FZO1B antibody

Mitofusin 1 antibody

Expand

Images

  • Western blot analysis of Mitofusin 1 on K-562 cell lysates with Rabbit anti-Mitofusin 1 antibody (<a href="/products/ET1702-01" style="font-weight: bold;text-decoration: underline;">ET1702-01</a>) at 1/2,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 84 kDa<br />Observed band size: 84 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-01" style="font-weight: bold;text-decoration: underline;">ET1702-01</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Mitofusin 1 on K-562 cell lysates with Rabbit anti-Mitofusin 1 antibody (ET1702-01) at 1/2,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 84 kDa
    Observed band size: 84 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-01) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation