PRMT5 Recombinant Rabbit Monoclonal Antibody [ST51-06]
Overview
Product Name
PRMT5 Recombinant Rabbit Monoclonal Antibody [ST51-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human PRMT5 aa 48-91 / 637.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Molecular Weight
Predicted band size: 73 kDa
Positive Control
HepG2 cell lysate, HEK-293 cell lysate, Raji cell lysate, Jurkat cell lysate, A431 cell lysate, C6 cell lysate, PC-12 cell lysate, bEnd.3 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, HepG2, MCF7 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, Neuro-2a, human kidney tissue, mouse ovary tissue, rat ovary tissue.
Conjugation
unconjugated
Clone Number
ST51-06
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000-1:5,000
-
IF-Cell
-
1:50-1:250
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:500-1:2,000
-
IP
-
1-2μg/sample
-
FC
-
1:1,000
Target
Function
The formation of the spliceosome includes the assembly of Sm proteins in an ordered manner onto snRNAs. This process is mediated by the survival of a motor neuron (SMN) protein and is enhanced by modification of specific Arginine residues in the Sm proteins to symmetrical dimethylarginines (sDMAs). sDMA modification of Sm proteins is catalyzed by the methylosome, a complex comprised of the type II methyltransferase PRMT5, also designated JAK-binding protein 1), (JBP1), pICln, and two novel factors. PRMT5 binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, while pICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes to the cytoplasm in a wide variety of tissues. PRMT5 also associates specifically with the transcription start site region of the cyclin E1 promoter, and, therefore, is involved in the control of transcription and proliferation. The gene encoding human PRMT5 maps to chromosome 14q11.
Background References
1. Gurung B et al. Menin directly represses gli1 expression independent of canonical hedgehog signaling. Mol Cancer Res 11:1215-22 (2013).
2. Goyal A et al. Generation of human induced pluripotent stem cells using epigenetic regulators reveals a germ cell-like identity in partially reprogrammed colonies. PLoS One 8:e82838 (2013).
Sequence Similarity
Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.
Tissue Specificity
Ubiquitous.
Subcellular Location
Cytoplasm, Nucleus, Golgi apparatus, Chromosome.
Synonyms
72 kDa ICln binding protein antibody
72 kDa ICln-binding protein antibody
ANM5_HUMAN antibody
Histone synthetic lethal 7, S. cerevisiae, homolog of antibody
Histone-arginine N-methyltransferase PRMT5 antibody
HMT1 hnRNP methyltransferase like 5 antibody
HOMOLOG OF
SKB1 antibody
HRMT1L5 antibody
IBP72 antibody
ExpandImages
-
Western blot analysis of PRMT5 on different lysates with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Raji cell lysate
Lane 4: Jurkat cell lysate
Lane 5: A431 cell lysate
Lane 6: C6 cell lysate
Lane 7: PC-12 cell lysate
Lane 8: bEnd.3 cell lysate
Lane 9: NIH/3T3 cell lysate
Lane 10: C2C12 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-43) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HepG2 cells labeling PRMT5 with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/250 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Western blot analysis of PRMT5 on different lysates with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Raji cell lysate
Lane 4: Jurkat cell lysate
Lane 5: MCF7 cell lysate
Lane 6: A431 cell lysate
Lane 7: COS-1 cell lysate
Lane 8: Neuro-2a cell lysate
Lane 9: bEnd.3 cell lysate
Lane 10: NIH/3T3 cell lysate
Lane 11: C2C12 cell lysate
Lane 12: C6 cell lysate
Lane 13: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 70 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-43) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Neuro-2a cells labeling PRMT5 with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-43) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-43) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-PRMT5 antibody (ET1609-43) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-43) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of HepG2 cells labeling PRMT5.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1609-43, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of Neuro-2a cells labeling PRMT5.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1609-43, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
PRMT5 was immunoprecipitated in 0.2mg HepG2 cell lysate with ET1609-43 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1609-43 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate (input)
Lane 2: ET1609-43 IP in HepG2 cell lysate
Lane 3: Rabbit IgG instead of ET1609-43 in HepG2 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Development of a Selective and Potent PRMT4 PROTAC Degrader with Efficacy against Multiple Myeloma in Vitro and in Vivo
Journal: Journal Of Medicinal Chemistry
DOI: 10.1021/acs.jmedchem.5c01022
IF: 6.8
Application: WB
Reactivity: Human
Publish date: 2025 Jun
-
Protein arginine methyltransferase 5 mediates arginine symmetric dimethylation of influenza A virus PB2 and supports viral replication
Journal: Journal Of Medical Virology
DOI:
IF: 12.7
Application: WB
Reactivity: Human
Publish date: 2023 Oct