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RRM1 Recombinant Rabbit Monoclonal Antibody [JB09-43]

Usd: 385

Specification

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Catalog# ET7107-72

RRM1 Recombinant Rabbit Monoclonal Antibody [JB09-43]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

RRM1 Recombinant Rabbit Monoclonal Antibody [JB09-43]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human RRM1 aa 651-792 / 792.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 90 kDa

Positive Control

A431 cell lysate, HeLa cell lysate, HT-29 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate, Rat spleen tissue lysate, human colon cancer tissue, human lymph node tissue, A431.

Conjugation

unconjugated

Clone Number

JB09-43

RRID

AB_3070781

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50

Target

Function

This gene encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1), with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene.

Background References

1. Qiu W et al. Characterization of enzymatic properties of human ribonucleotide reductase holoenzyme reconstituted in vitro from hRRM1, hRRM2, and p53R2 subunits. Biochem Biophys Res Commun 340:428-434 (2006).

2. Harrington J A et al. Human ribonucleotide reductase. Activation and inhibition by analogs of ATP. Biochem Pharmacol 42:759-763 (1991).

Sequence Similarity

Belongs to the ribonucleoside diphosphate reductase large chain family.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P23921 Human

SwissProt: P07742 Mouse

Entrez Gene: 685579 Rat

Synonyms

R1 antibody

Ribonucleoside diphosphate reductase large subunit antibody

Ribonucleoside diphosphate reductase M1 chain antibody

Ribonucleoside diphosphate reductase subunit M1 antibody

Ribonucleoside reductase, large subunit antibody

Ribonucleoside-diphosphate reductase large subunit antibody

Ribonucleoside-diphosphate reductase subunit M1 antibody

Ribonucleotide reductase large chain antibody

Ribonucleotide reductase large subunit antibody

Ribonucleotide reductase M1 antibody

Expand

Images

  • Western blot analysis of RRM1 on different lysates with Rabbit anti-RRM1 antibody (<a href="/products/ET7107-72" style="font-weight: bold;text-decoration: underline;">ET7107-72</a>) at 1/1,000 dilution.<br /><br />Lane 1: A431 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: HT-29 cell lysate<br />Lane 4: Mouse thymus tissue lysate<br />Lane 5: Rat thymus tissue lysate<br />Lane 6: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 90 kDa<br />Observed band size: 90 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-72" style="font-weight: bold;text-decoration: underline;">ET7107-72</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RRM1 on different lysates with Rabbit anti-RRM1 antibody (ET7107-72) at 1/1,000 dilution.

    Lane 1: A431 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: HT-29 cell lysate
    Lane 4: Mouse thymus tissue lysate
    Lane 5: Rat thymus tissue lysate
    Lane 6: Rat spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 90 kDa
    Observed band size: 90 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-72) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-RRM1 antibody (<a href="/products/ET7107-72" style="font-weight: bold;text-decoration: underline;">ET7107-72</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-72" style="font-weight: bold;text-decoration: underline;">ET7107-72</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-RRM1 antibody (ET7107-72) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-72) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-RRM1 antibody (<a href="/products/ET7107-72" style="font-weight: bold;text-decoration: underline;">ET7107-72</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7107-72" style="font-weight: bold;text-decoration: underline;">ET7107-72</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-RRM1 antibody (ET7107-72) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-72) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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