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RbAP48 Recombinant Rabbit Monoclonal Antibody [JU37-47]

Usd: 385

Specification

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Catalog# ET1706-54

RbAP48 Recombinant Rabbit Monoclonal Antibody [JU37-47]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

RbAP48 Recombinant Rabbit Monoclonal Antibody [JU37-47]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human RbAP48 aa 1-98 / 425.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, FC, IP

Molecular Weight

48 kDa

Positive Control

NIH-3T3 cell lysate, Hela cell lysate, rat brain tissue lysate, mouse testis tissue lysate, Hela, MCF-7, SH-SY5Y, rat brain tissue, human tonsil tissue, mouse testis tissue, human lung carcinoma tissue, human liver tissue,Hela.

Conjugation

unconjugated

Clone Number

JU37-47

RRID

AB_3070674

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

Target

Function

In the intact cell, DNA is closely associated with histones and other nuclear proteins to form chromatin.The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation, and an increased accessiblity of DNA to transcription factors. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. The retinoblastoma binding proteins RbAp46 and RbAp48 have been identified as histone binding proteins, and they are components of the histone deacetylase complex.

Background References

1. Zhang Q et al. Histone binding protein RbAp48 interacts with a complex of CREB binding protein and phosphorylated CREB. Mol Cell Biol 20:4970-4978 (2000).

2. Verreault A et al. Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4. Cell 87:95-104 (1996).

Sequence Similarity

Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q09028 Human

SwissProt: Q60972 Mouse

Synonyms

CAF I p48 antibody

CAF-1 subunit C antibody

CAF-I 48 kDa subunit antibody

CAF-I p48 antibody

Chromatin assembly factor 1 subunit C antibody

Chromatin assembly factor I p48 subunit antibody

Chromatin assembly factor/CAF 1 p48 subunit antibody

Histone-binding protein RBBP4 antibody

MSI1 protein homolog antibody

Nucleosome-remodeling factor subunit RBAP48 antibody

Expand

Images

  • Western blot analysis of RbAP48 on different lysates with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/500 dilution.<br /> <br />Lane 1: NIH-3T3 cell lysates, 10 µg/Lane<br />Lane 2: Hela cell lysates, 10 µg/Lane<br />Lane 3: Rat brain tissue lysates, 20 µg/Lane<br />Lane 4: Mouse testis tissue lysates, 20 µg/Lane<br /><br />Predicted band size: 48 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RbAP48 on different lysates with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/500 dilution.

    Lane 1: NIH-3T3 cell lysates, 10 µg/Lane
    Lane 2: Hela cell lysates, 10 µg/Lane
    Lane 3: Rat brain tissue lysates, 20 µg/Lane
    Lane 4: Mouse testis tissue lysates, 20 µg/Lane

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-54) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Hela cells labeling RbAP48 with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Hela cells labeling RbAP48 with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of MCF-7 cells labeling RbAP48 with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of MCF-7 cells labeling RbAP48 with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling RbAP48 with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of SH-SY5Y cells labeling RbAP48 with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-54) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-54) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-54) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-54) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-RbAP48 antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1706-54" style="font-weight: bold;text-decoration: underline;">ET1706-54</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-RbAP48 antibody (ET1706-54) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-54) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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