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SAE2 / UBA2 Recombinant Rabbit Monoclonal Antibody [JM66-53]

Usd: 385

Specification

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Catalog# ET1705-73

SAE2 / UBA2 Recombinant Rabbit Monoclonal Antibody [JM66-53]

Application

  • WB

  • IP

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SAE2 / UBA2 Recombinant Rabbit Monoclonal Antibody [JM66-53]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human SAE2 / UBA2 aa 39-88 / 640.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP, IHC-P

Molecular Weight

Predicted band size: 71 kDa

Positive Control

HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, K-562 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse colon tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

JM66-53

RRID

AB_3070603

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IP

  • 1:10-1:50

  • IHC-P

  • 1:50-1:200

Target

Function

The small ubiquitin-related modifier protein SUMO-1 belongs to the ubiquitin-like protein family, which are synthesized as precursor proteins that undergo processing before conjugation to target proteins. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processes, including nuclear transport, transcriptional regulation, apoptosis, and protein stability. SUMO-1 utilizes homologues of the E1 and E2 enzymes for conjugation to proteins, which include IkBα, MDM2, p53, PML, and RanGap1. AOS1 is homologous to the N-terminal half of E1 and UBA2 is homologous to the C-terminal half of E1. These proteins form a heterodimer that activates SUMO-1.

Background References

1. Qin, Y.et al. SUMOylation alterations are associated with multidrug resistance in hepatocellular carcinoma. Mol Med Rep. 9: 877-81 (2014).

2. Du L et al. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal. Nat Commun 7:12326 (2016).

Sequence Similarity

Belongs to the ubiquitin-activating E1 family.

Post-translational Modification

Sumoylated with SUMO1 and SUMO2/3 and by UBC9. Sumoylation at Lys-236 inhibits enzymatic activity. Sumoylation at the C-terminal lysine cluster plays an essential role in nuclear trafficking.

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q9UBT2 Human

SwissProt: Q9Z1F9 Mouse

SwissProt: B1WC32 Rat

Synonyms

Anthracycline associated resistance ARX antibody

Anthracycline-associated resistance ARX antibody

ARX antibody

FLJ13058 antibody

HRIHFB2115 antibody

SAE 2 antibody

SAE2 antibody

SAE2_HUMAN antibody

SUMO 1 activating enzyme subunit 2 antibody

SUMO activating enzyme subunit 2 antibody

Expand

Images

  • Western blot analysis of SAE2 / UBA2 on different lysates with Rabbit anti-SAE2 / UBA2 antibody (<a href="/products/ET1705-73" style="font-weight: bold;text-decoration: underline;">ET1705-73</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: HT-29 cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: RAW264.7 cell lysate<br />Lane 6: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 71 kDa<br />Observed band size: 90 kDa<br /><br />Exposure time: 13 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-73" style="font-weight: bold;text-decoration: underline;">ET1705-73</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SAE2 / UBA2 on different lysates with Rabbit anti-SAE2 / UBA2 antibody (ET1705-73) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate
    Lane 3: HT-29 cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: RAW264.7 cell lysate
    Lane 6: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 71 kDa
    Observed band size: 90 kDa

    Exposure time: 13 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-73) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SAE2 / UBA2 antibody (<a href="/products/ET1705-73" style="font-weight: bold;text-decoration: underline;">ET1705-73</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-73" style="font-weight: bold;text-decoration: underline;">ET1705-73</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-SAE2 / UBA2 antibody (ET1705-73) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-SAE2 / UBA2 antibody (<a href="/products/ET1705-73" style="font-weight: bold;text-decoration: underline;">ET1705-73</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-73" style="font-weight: bold;text-decoration: underline;">ET1705-73</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-SAE2 / UBA2 antibody (ET1705-73) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-73) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation