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SEC23 Recombinant Rabbit Monoclonal Antibody [JU37-48]

Usd: 385

Specification

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Catalog# ET7106-78

SEC23 Recombinant Rabbit Monoclonal Antibody [JU37-48]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

SEC23 Recombinant Rabbit Monoclonal Antibody [JU37-48]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human SEC23 aa 280-420 / 765.

Species Reactivity

Human, Mouse

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

86/63 kDa

Positive Control

NIH-3T3, HepG2, HUVEC, human tonsil tissue, human fetal skeletal muscle tissue, HepG2.

Conjugation

unconjugated

Clone Number

JU37-48

RRID

AB_3070698

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.

Background References

1. Paccaud J P et al. Cloning and functional characterization of mammalian homologues of the COPII component Sec23. Mol Biol Cell 7:1535-1546 (1996).

2. Mancias J D et al. The transport signal on Sec22 for packaging into COPII-coated vesicles is a conformational epitope. Mol Cell 26:403-414 (2007).

Sequence Similarity

Belongs to the SEC23/SEC24 family. SEC23 subfamily.

Tissue Specificity

Ubiquitously expressed.

Subcellular Location

Cytosol. Endoplasmic reticulum.

UNIPROT

SwissProt: Q15436 Human

SwissProt: Q01405 Mouse

Synonyms

CLSD antibody

Protein transport protein Sec23A antibody

SC23A_HUMAN antibody

Sec23 homolog A (S. cerevisiae) antibody

SEC23-related protein A antibody

sec23a antibody

Images

  • Western blot analysis of SEC23 on NIH-3T3 cell using anti-SEC23 antibody at 1/500 dilution.

    Western blot analysis of SEC23 on NIH-3T3 cell using anti-SEC23 antibody at 1/500 dilution.

  • ICC staining SEC23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining SEC23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining SEC23 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining SEC23 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SEC23 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SEC23 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SEC23 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SEC23 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"