SNF5 Recombinant Rabbit Monoclonal Antibody [JB49-18]
Catalog# ET7107-84
SNF5 Recombinant Rabbit Monoclonal Antibody [JB49-18]
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SNF5 Recombinant Rabbit Monoclonal Antibody [JB49-18]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide of C terminal Human SNF5.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 44 kDa
Positive Control
Daudi cell lysate, PC-12 cell lysate, Human lung tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, NIH/3T3.
Conjugation
unconjugated
Clone Number
JB49-18
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
Target
Function
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
Background References
1. Morozov A et al. Structure-function analysis of integrase interactor 1/hSNF5L1 reveals differential properties of two repeat motifs present in the highly conserved region. Proc Natl Acad Sci USA 95:1120-1125 (1998).
2. Versteege I et al. A key role of the hSNF5/INI1 tumour suppressor in the control of the G1-S transition of the cell cycle. Oncogene 21:6403-6412 (2002).
Sequence Similarity
Belongs to the SNF5 family.
Subcellular Location
Nucleus.
Synonyms
BAF47 antibody
BRG1-associated factor 47 antibody
hSNF5 antibody
INI1 antibody
Integrase interactor 1 protein antibody
Malignant rhabdoid tumor suppressor antibody
RDT antibody
RTPS1 antibody
Sfh1p antibody
SMARCB1 antibody
ExpandImages
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Western blot analysis of SNF5 on different lysates with Rabbit anti-SNF5 antibody (ET7107-84) at 1/1,000 dilution.
Lane 1: Daudi cell lysate (20 µg/Lane)
Lane 2: PC-12 cell lysate (20 µg/Lane)
Lane 3: Human lung tissue lysate (40 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 44 kDa
Observed band size: 44 kDa
Exposure time: Lane 1: 20 seconds; Lane 2-5: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"