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TGFBI Recombinant Rabbit Monoclonal Antibody [JM24-53]

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Specification

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Catalog# ET1705-29

TGFBI Recombinant Rabbit Monoclonal Antibody [JM24-53]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TGFBI Recombinant Rabbit Monoclonal Antibody [JM24-53]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human TGFBI aa 93-142 / 683.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 75 kDa

Positive Control

Mouse small intestine tissue lysate, Mouse colon tissue lysate, Rat colon tissue lysate, Rat uterus tissue lysate, human colon tissue, human colon cancer tissue.

Conjugation

unconjugated

Clone Number

JM24-53

RRID

AB_3070560

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene, locus 5q31. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.

Background References

1. Huang AH et al. Evaluation of the complex transcriptional topography of mesenchymal stem cell chondrogenesis for cartilage tissue engineering. Tissue Eng Part A 16:2699-708 (2010).

2. He Y et al. Largescale Transcriptomics Analysis Suggests Over-Expression of BGH3, MMP9 and PDIA3 in Oral Squamous Cell Carcinoma. PLoS One 11:e0146530 (2016).

Tissue Specificity

Highly expressed in the corneal epithelium. Expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Post-translational Modification

Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; these residues may be required for binding to calcium. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues.; The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain.

Subcellular Location

Secreted, extracellular space, extracellular matrix.

UNIPROT

SwissProt: Q15582 Human

SwissProt: P82198 Mouse

SwissProt: D4A8G5 Rat

Synonyms

RGD containing collagen associated protein antibody

AI181842 antibody

AI747162 antibody

Beta ig antibody

Beta ig h3 antibody

Beta ig-h3 antibody

BGH3_HUMAN antibody

Big h3 antibody

BIGH3 antibody

CDB1 antibody

Expand

Images

  • Western blot analysis of TGFBI on different lysates with Rabbit anti-TGFBI antibody (<a href="/products/ET1705-29" style="font-weight: bold;text-decoration: underline;">ET1705-29</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse small intestine tissue lysate (20 µg/Lane)<br />Lane 2: Mouse colon tissue lysate (20 µg/Lane)<br />Lane 3: Rat colon tissue lysate (20 µg/Lane)<br />Lane 4: Rat uterus tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 75 kDa<br />Observed band size: 68 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1705-29" style="font-weight: bold;text-decoration: underline;">ET1705-29</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TGFBI on different lysates with Rabbit anti-TGFBI antibody (ET1705-29) at 1/1,000 dilution.

    Lane 1: Mouse small intestine tissue lysate (20 µg/Lane)
    Lane 2: Mouse colon tissue lysate (20 µg/Lane)
    Lane 3: Rat colon tissue lysate (20 µg/Lane)
    Lane 4: Rat uterus tissue lysate (20 µg/Lane)

    Predicted band size: 75 kDa
    Observed band size: 68 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-29) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TGFBI antibody (<a href="/products/ET1705-29" style="font-weight: bold;text-decoration: underline;">ET1705-29</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-29" style="font-weight: bold;text-decoration: underline;">ET1705-29</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-TGFBI antibody (ET1705-29) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-29) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-TGFBI antibody (<a href="/products/ET1705-29" style="font-weight: bold;text-decoration: underline;">ET1705-29</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-29" style="font-weight: bold;text-decoration: underline;">ET1705-29</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-TGFBI antibody (ET1705-29) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-29) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation