Skip to content

TTC11/FIS1 Recombinant Rabbit Monoclonal Antibody [JE40-90]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET7109-17

TTC11/FIS1 Recombinant Rabbit Monoclonal Antibody [JE40-90]

Application

  • WB

  • IF-Cell

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TTC11/FIS1 Recombinant Rabbit Monoclonal Antibody [JE40-90]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human TTC11 aa 1-125 / 152.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 17 kDa

Positive Control

Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate, SK-Br-3 cell lysate, MCF7 cell lysate, SK-OV-3 cell lysateMCF-7, SH-SY-5Y, SK-Br-3, human kidney tissue, human small intestine tissue, human uterus tissue, mouse brain tissue, mouse colon tissue, rat brain tissue, rat colon tissue.

Conjugation

unconjugated

Clone Number

JE40-90

RRID

AB_3070907

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:200

Target

Function

Fis1 localizes to the outer mitochondrial membrane and, along with dynamin-related protein (Drp1), participates in mitochondrial fission. Fission and fusion mechanisms regulate mitochondrial morphology within the cell. Fission frequency is determined by the level of Fis1 molecules at the mitochondrial surface. Fis1 contains a C-terminal domain, which is required for mitochondrial localization, and an N-terminal domain, which is necessary for mitochondrial fission. Fragmentation of the mitochondrial network by Fis1 leads to cytochrome c release and apoptosis. The mitochondrial fission mechanisms may be involved in positively and negatively regulating apoptosis.

Background References

1. James D I et al. hFis1, a novel component of the mammalian mitochondrial fission machinery. J Biol Chem 278:36373-36379 (2003).

2. Yoon Y et al. The mitochondrial protein hFis1 regulates mitochondrial fission in mammalian cells through an interaction with the dynamin-like protein DLP1. Mol Cell Biol 23:5409-5420 (2003).

Sequence Similarity

Belongs to the FIS1 family.

Post-translational Modification

Ubiquitinated by MARCHF5.

Subcellular Location

Peroxisome. Mitochondrion.

UNIPROT

SwissProt: Q9Y3D6 Human

SwissProt: Q9CQ92 Mouse

SwissProt: P84817 Rat

Synonyms

2010003O14Rik antibody

CGI 135 antibody

CGI135 antibody

FIS 1 antibody

FIS1 antibody

Fis1 homolog antibody

FIS1, S. cerevisiae, homolog of antibody

FIS1_HUMAN antibody

Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) antibody

Fission 1 (mitochondrial outer membrane) homolog (yeast) antibody

Expand

Images

  • Western blot analysis of TTC11/FIS1 on different lysates with Rabbit anti-TTC11/FIS1 antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Mouse brain tissue lysate<br />Lane 3: Rat spleen tissue lysate<br />Lane 4: Rat brain tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 17 kDa<br />Observed band size: 15 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TTC11/FIS1 on different lysates with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/1,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Mouse brain tissue lysate
    Lane 3: Rat spleen tissue lysate
    Lane 4: Rat brain tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 17 kDa
    Observed band size: 15 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of TTC11/FIS1 on different lysates with Rabbit anti-TTC11/FIS1 antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/2,000 dilution.<br /><br />Lane 1: SK-Br-3 cell lysate<br />Lane 2: MCF7 cell lysate<br />Lane 3: SK-OV-3 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 17 kDa<br />Observed band size: 17 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TTC11/FIS1 on different lysates with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/2,000 dilution.

    Lane 1: SK-Br-3 cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: SK-OV-3 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

    Exposure time: 2 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-17) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining TTC11 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TTC11 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining TTC11 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TTC11 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining TTC11 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining TTC11 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TTC11/FIS1 antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-TTC11/FIS1 antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TTC11/FIS1 antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-TTC11/FIS1 antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7109-17" style="font-weight: bold;text-decoration: underline;">ET7109-17</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-TTC11/FIS1 antibody (ET7109-17) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation