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Transferrin Receptor 2 Recombinant Rabbit Monoclonal Antibody [JG58-34]

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Specification

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Catalog# ET7108-21

Transferrin Receptor 2 Recombinant Rabbit Monoclonal Antibody [JG58-34]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Transferrin Receptor 2 Recombinant Rabbit Monoclonal Antibody [JG58-34]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Transferrin Receptor 2 aa 130-320 / 801.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 89 kDa

Positive Control

K562 cell lysates, human liver tissue, human liver carcinoma tissue.

Conjugation

unconjugated

Clone Number

JG58-34

RRID

AB_3070821

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:1,000

Target

Function

Iron is a vital molecule for living organisms because it is involved in a wide variety of metabolic processes, such as oxygen transport, DNA synthesis and electron transport. Excessive iron uptake leads to tissue damage as a result of formation of free radicals. Iron uptake and storage is tightly regulated by the feedback system of iron responsive element-containing gene products and iron regulatory proteins that modulate the expression levels of the genes involved in iron metabolism. The transferrin receptor 2 (TFR2) mediates the uptake of transferrin-bound iron. It is involved in iron metabolism, hepatocyte function and erythrocyte differentiation, and is highly expressed as a protein in liver as well as in hepatocytes and erythroid precursors. The gene encoding human TRF2 maps to chromosome 7q22 and is expressed as an a isoform, which encodes a transmembrane protein, and a b isoform, which encodes a shorter, intracellular protein. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder that results in clinical complications, including cirrhosis, cardiopathy, diabetes, endocrine dysfunctions, arthropathy and susceptibility to liver cancer.

Background References

1. Roetto A et al. New mutations inactivating transferrin 2 in hemochromatosis type 3. Blood 97:2555-2560 (2001).

2. Mattman A et al. Transferrin receptor 2 (TfR2) and HFE mutational analysis in non-C282Y iron overload: identification of a novel TfR2 mutation. Blood 100:1075-1077 (2002).

Sequence Similarity

Belongs to the peptidase M28 family. M28B subfamily.

Tissue Specificity

Predominantly expressed in liver. While the alpha form is also expressed in spleen, lung, muscle, prostate and peripheral blood mononuclear cells, the beta form is expressed in all tissues tested, albeit weakly.

Subcellular Location

Cell membrane. Cytoplasm.

UNIPROT

SwissProt: Q9UP52 Human

Synonyms

HFE 3 antibody

HFE3 antibody

MGC126368 antibody

TFR 2 antibody

TfR2 antibody

TFR2_HUMAN antibody

TFRC 2 antibody

TFRC2 antibody

Transferrin receptor 2 antibody

Transferrin receptor protein 2 antibody

Images

  • Western blot analysis of Transferrin Receptor 2 on K562 cell lysates with Rabbit anti-Transferrin Receptor 2 antibody (<a href="/products/ET7108-21" style="font-weight: bold;text-decoration: underline;">ET7108-21</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 89 kDa<br />Observed band size: 89 kDa<br /><br />Exposure time: 2 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7108-21" style="font-weight: bold;text-decoration: underline;">ET7108-21</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Transferrin Receptor 2 on K562 cell lysates with Rabbit anti-Transferrin Receptor 2 antibody (ET7108-21) at 1/500 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 89 kDa
    Observed band size: 89 kDa

    Exposure time: 2 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-21) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Transferrin Receptor 2 antibody (<a href="/products/ET7108-21" style="font-weight: bold;text-decoration: underline;">ET7108-21</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-21" style="font-weight: bold;text-decoration: underline;">ET7108-21</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Transferrin Receptor 2 antibody (ET7108-21) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-21) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Transferrin Receptor 2 antibody (<a href="/products/ET7108-21" style="font-weight: bold;text-decoration: underline;">ET7108-21</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7108-21" style="font-weight: bold;text-decoration: underline;">ET7108-21</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-Transferrin Receptor 2 antibody (ET7108-21) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-21) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation