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VPS26 Recombinant Rabbit Monoclonal Antibody [JB93-41]

Usd: 385

Specification

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Catalog# ET7107-21

VPS26 Recombinant Rabbit Monoclonal Antibody [JB93-41]

Application

  • WB

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

VPS26 Recombinant Rabbit Monoclonal Antibody [JB93-41]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human VPS26 aa 210-327 / 327.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP

Molecular Weight

Predicted band size: 38 kDa

Positive Control

Human liver tissue lysate, Hela cell lysate, rat kidney tissue, human tonsil tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

JB93-41

RRID

AB_3070736

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50

Target

Function

Vacuolar protein sorting-associated protein 26A is a protein that in humans is encoded by the VPS26A gene. This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Background References

1. Harbour M.E. et al. The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics. J. Cell Sci. 123:3703-3717(2010).

2. Steinberg F. et al. A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Nat. Cell Biol. 15:461-471(2013).

Sequence Similarity

Belongs to the VPS26 family.

Subcellular Location

Endosome. Cytoplasm.

UNIPROT

SwissProt: O75436 Human

SwissProt: P40336 Mouse

SwissProt: Q6AY86 Rat

Synonyms

FLJ12930 antibody

HB 58 antibody

HB58 antibody

Hbeta 58 antibody

Hbeta 58 homolog antibody

Hbeta58 antibody

Hbeta58 homolog antibody

hVPS 26 antibody

hVPS26 antibody

MGC94168 antibody

Expand

Images

  • Western blot analysis of VPS26 on different lysates with Rabbit anti-VPS26 antibody (<a href="/products/ET7107-21" style="font-weight: bold;text-decoration: underline;">ET7107-21</a>) at 1/500 dilution.<br /><br />Lane 1: Human liver tissue lysate (20 µg/Lane)<br />Lane 2: Hela cell lysate (10 µg/Lane)<br /><br />Predicted band size: 38 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-21" style="font-weight: bold;text-decoration: underline;">ET7107-21</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of VPS26 on different lysates with Rabbit anti-VPS26 antibody (ET7107-21) at 1/500 dilution.

    Lane 1: Human liver tissue lysate (20 µg/Lane)
    Lane 2: Hela cell lysate (10 µg/Lane)

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-21) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of VPS26 on different lysates with Rabbit anti-VPS26 antibody (<a href="/products/ET7107-21" style="font-weight: bold;text-decoration: underline;">ET7107-21</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-VPS26 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 38 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7107-21" style="font-weight: bold;text-decoration: underline;">ET7107-21</a>) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of VPS26 on different lysates with Rabbit anti-VPS26 antibody (ET7107-21) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-VPS26 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-21) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-VPS26 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-VPS26 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VPS26 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-VPS26 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-VPS26 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-VPS26 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"