mGluR1 Recombinant Rabbit Monoclonal Antibody [JM11-61]
Catalog# ET1703-84
mGluR1 Recombinant Rabbit Monoclonal Antibody [JM11-61]
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WB
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IHC-P
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IF-Tissue
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IHC-Fr
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
mGluR1 Recombinant Rabbit Monoclonal Antibody [JM11-61]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human mGluR1 aa 280-420 / 1194.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue, IHC-Fr
Target Molecular Weight
Predicted band size: 132 kDa
Positive Control
Mouse cerebellum tissue lysate, rat brain tissue lysate, rat cerebellum tissue lysate, mouse brain tissue, mouse kidney tissue, rat cerebellum tissue, mouse cerebellum tissue, human cerebellum tissue.
Conjugation
unconjugated
Clone Number
JM11-61
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:1,000
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IF-Tissue
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1:50-1:200
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IHC-Fr
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1:200
Target
Function
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
Background References
1. Valiya Veettil M et al. Glutamate secretion and metabotropic glutamate receptor 1 expression during Kaposi\'s sarcoma-associated herpesvirus infection promotes cell proliferation. PLoS Pathog 10:e1004389 (2014).
2. Sylantyev S et al. Spike-driven glutamate electrodiffusion triggers synaptic potentiation via a homer-dependent mGluR-NMDAR link. Neuron 77:528-41 (2013).
Sequence Similarity
Belongs to the G-protein coupled receptor 3 family.
Tissue Specificity
Detected in brain.
Subcellular Location
Cell membrane.
Synonyms
Glutamate receptor metabotropic 1 antibody
glutamate receptor, metabotropic 1 antibody
GPRC1A antibody
GRM1 antibody
GRM1-Alpha antibody
GRM1_HUMAN antibody
GRM1A antibody
Metabotropic glutamate receptor 1 antibody
mGlu1 antibody
mGluR1 antibody
ExpandImages
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Western blot analysis of mGluR1 on different lysates with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/1,000 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Rat cerebellum tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 132 kDa
Observed band size: 300 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-84) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-84) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-84) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-84) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-84) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-84) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-mGluR1 antibody (ET1703-84) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-84, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"