Renin Recombinant Rabbit Monoclonal Antibody [PSH19-51]
Catalog# HA724077
Renin Recombinant Rabbit Monoclonal Antibody [PSH19-51]
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WB
-
IHC-P
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Human
-
unconjugated
Overview
Product Name
Renin Recombinant Rabbit Monoclonal Antibody [PSH19-51]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 45 kDa
Positive Control
HT-29 cell lysates, human kideny tissue.
Conjugation
unconjugated
Clone Number
PSH19-51
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
|---|---|---|---|---|
| human |
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|
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
-
1:1,000
Target
Function
Renin, also known as an angiotensinogenase, is an aspartic protease protein and enzyme secreted by the kidneys that participates in the body's renin-angiotensin-aldosterone system (RAAS)—also known as the renin-angiotensin-aldosterone axis—that increases the volume of extracellular fluid (blood plasma, lymph, and interstitial fluid) and causes arterial vasoconstriction. Thus, it increases the body's mean arterial blood pressure. Renin is not commonly referred to as a hormone, although it has a receptor, the (pro)renin receptor, also known as the renin receptor and prorenin receptor, as well as enzymatic activity with which it hydrolyzes angiotensinogen to angiotensin I.
Background References
1. Yamaguchi H et al. Renin Cells, From Vascular Development to Blood Pressure Sensing. Hypertension. 2023 Aug
2. Sequeira-Lopez MLS et al. Renin Cells, the Kidney, and Hypertension. Circ Res. 2021 Apr
Subcellular Location
Secreted, Membrane.
UNIPROT
Synonyms
Angiotensin forming enzyme antibody
Angiotensin forming enzyme precursor antibody
Angiotensinogenase antibody
Angiotensinogenase precursor antibody
FLJ10761 antibody
HNFJ2 antibody
REN antibody
Ren1 antibody
RENI_HUMAN antibody
Renin antibody
ExpandImages
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Western blot analysis of Renin on HT-29 cell lysates with Rabbit anti-Renin antibody (HA724077) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724077) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kideny tissue with Rabbit anti-Renin antibody (HA724077) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA724077) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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