SARS-CoV-2 Nucleocapsid Protein Mouse Monoclonal Antibody [A4D12]
Catalog# EM1902-21
SARS-CoV-2 Nucleocapsid Protein Mouse Monoclonal Antibody [A4D12]
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ELISA
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WB
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SARS-CoV-2
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unconjugated
Overview
Product Name
SARS-CoV-2 Nucleocapsid Protein Mouse Monoclonal Antibody [A4D12]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant SARS-CoV-2 Nucleocapsid Protein.
Species Reactivity
SARS-CoV-2
Validated Applications
ELISA, WB
Target Molecular Weight
46 kDa
Positive Control
ELISA: Recombinant SARS-CoV-2 Nucleocapsid Protein. WB: N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein and inactivated native SARS-CoV-2 lysate.
Conjugation
unconjugated
Clone Number
A4D12
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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ELISA
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Use at an assay dependent concentration.
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WB
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1:500-1:5,000
Target
Function
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Background References
暂无
Sequence Similarity
Belongs to the betacoronavirus nucleocapsid protein family.
Post-translational Modification
ADP-ribosylated. The ADP-ribosylation is retained in the virion during infection.; Phosphorylated on serine and threonine residues.
Subcellular Location
Virion, Host endoplasmic reticulum-Golgi intermediate compartment, Host Golgi apparatus.
UNIPROT
Synonyms
SARS-CoV-2 Nucleocapsid Protein antibody
SARS-CoV-2 NP antibody
nucleocapsid protein antibody
novel coronavirus N Protein antibody
novel coronavirus Nucleocapsid Protein antibody
2019-nCoV Nucleoprotein antibody
2019-nCoV N antibody
2019nCoV N antibody
2019-nCoV N Protein antibody
2019 peak N Protein antibody
ExpandImages
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The binding activity of EM1902-21 with SARS-CoV-2 Recombinant Nucleocapsid protein.
Immobilized SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (EM1902-21) for 1 hour at 25℃. The EC50 of EM1902-21 is 271.3 ng/ml. -
Western blot analysis of EM1902-21 with N-terminal His tag-tagged SARS-CoV-2 Nucleocapsid protein (1) and Inactivated SARS-CoV-2 lysate (2) at 20ng.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1902-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"