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SCAI Mouse Monoclonal Antibody [7-A4]

Usd: 350

Specification

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Catalog# M1004-5

SCAI Mouse Monoclonal Antibody [7-A4]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

SCAI Mouse Monoclonal Antibody [7-A4]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein within Human SCAI aa 1-606 / 606.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 70 kDa

Positive Control

Recombinant protein, SHG-44, SH-SY-5Y, PC-12, human tonsil tissue, human lung tissue, human liver tissue, human brain tissue, human heart tissue, human pancreas tissue, human colon tissue

Conjugation

unconjugated

Clone Number

7-A4

RRID

AB_3073010

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgM

Purification Method

Protein L affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:400

Target

Function

Protein SCAI, also known as Suppressor of cancer cell invasion protein, and encoded by the gene name SCAI, is a tumor suppressor that suppresses MKL1-induced SRF transcriptional activity. It is a regulator of cell migration and appears to be involved in the RhoA (ras homolog gene family, member A)-Dia1 (diaphanous homolog 1) signal transduction pathway. SCAI localizes in the nucleus, where it binds and inhibits the myocardin-related transcription factor MAL by forming a ternary complex with serum response factor (SRF).

Background References

1. Klein AJ et al. SCAI appropriate use criteria for peripheral arterial interventions: An update. Catheter Cardiovasc Interv (2017).

2. Seto AH et al. Late breaking trials of 2016 in coronary artery disease: Commentary covering SCAI, ACC, TCT, EuroPCR, ESC, and AHA. Catheter Cardiovasc Interv. 89(6):1028-1034 (2017).

Sequence Similarity

Belongs to the SCAI family.

Subcellular Location

Membrane. Nucleus. Cytoplasm.

UNIPROT

SwissProt: Q8N9R8 Human

Synonyms

C9orf126 antibody

FLJ11758 antibody

FLJ36664 antibody

MGC120525 antibody

MGC120526 antibody

MGC120528 antibody

NET40 antibody

Protein SCAI antibody

SCAI antibody

SCAI_HUMAN antibody

Expand

Images

  • Western blot analysis of SCAI on Recombinant protein using anti-SCAI antibody at 1/1,000 dilution.

    Western blot analysis of SCAI on Recombinant protein using anti-SCAI antibody at 1/1,000 dilution.

  • ICC staining SCAI in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining SCAI in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining SCAI in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining SCAI in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining SCAI in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining SCAI in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SCAI antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SCAI antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-SCAI antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-SCAI antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SCAI antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SCAI antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-SCAI antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-SCAI antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-SCAI antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-SCAI antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-SCAI antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-SCAI antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-SCAI antibody (<a href="/products/M1004-5" style="font-weight: bold;text-decoration: underline;">M1004-5</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/M1004-5" style="font-weight: bold;text-decoration: underline;">M1004-5</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Mouse anti-SCAI antibody (M1004-5) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1004-5) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"