SFRP1 Rabbit Polyclonal Antibody
Catalog# ER1802-34
SFRP1 Rabbit Polyclonal Antibody
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
SFRP1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within N-terminal human SFRP1.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
Predicted band size: 35 kDa
Positive Control
A549, SH-SY-5Y, rat testis tissue, human colon cancer tissue, human breast tissue, human placenta tissue, mouse small intestine tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
-
IF-Cell
-
1:100-1:500
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
Target
Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
Background References
1. Liu L et al. FOS-driven inflammatory CAFs promote colorectal cancer liver metastasis via the SFRP1-FGFR2-HIF1 axis. Theranostics. 2025 Mar
2. Burgy O et al. Fibroblast-derived extracellular vesicles contain SFRP1 and mediate pulmonary fibrosis. JCI Insight. 2024 Aug
Sequence Similarity
Belongs to the secreted frizzled-related protein (sFRP) family.
Tissue Specificity
Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
Subcellular Location
Secreted.
Synonyms
Frizzled related protein 1 antibody
FRP 1 antibody
FRP antibody
FRP-1 antibody
FRP1 antibody
FrzA antibody
SARP 2 antibody
SARP-2 antibody
SARP2 antibody
Secreted apoptosis related protein 2 antibody
ExpandImages
-
ICC staining SFRP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
ICC staining SFRP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
-
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
-
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-SFRP1 antibody. Counter stained with hematoxylin.
-
Western blot analysis of SFRP1 on different lysates with Rabbit anti-SFRP1 antibody (ER1802-34) at 1/1,000 dilution.
Lane 1: SH-SY5Y cell lysate （20 µg/Lane）
Lane 2: SK-MEL-28 cell lysate（20 µg/Lane）
Lane 3: NIH/3T3 cell lysate（20 µg/Lane）
Lane 4: mouse kidney tissue lysate（40 µg/Lane）
Lane 5: mouse testis tissue lysate（40 µg/Lane）
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1802-34, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 35.4 kDa
Observed band size: 35 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
