SFT Recombinant Rabbit Monoclonal Antibody [JE64-46]
Catalog# HA722578
SFT Recombinant Rabbit Monoclonal Antibody [JE64-46]
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WB
-
IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SFT Recombinant Rabbit Monoclonal Antibody [JE64-46]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SFT aa 98-147 / 147.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Molecular Weight
Predicted band size: 17 kDa
Positive Control
Jurkat cell lysate, K-562 cell lysate, Raji cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human kidney tissue, human thyroid gland carcinoma tissue.
Conjugation
unconjugated
Clone Number
JE64-46
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000-1:2,000
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IP
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1-2μg/sample
Target
Function
Ubiquitin-conjugating enzyme E2 D1 is a protein that in humans is encoded by the UBE2D1 gene. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.
Background References
1. Guan XQ et al. IGF2BP2-modified UBE2D1 interacts with Smad2/3 to promote the progression of breast cancer. Am J Cancer Res. 2023 Jul
2. Wang X et al. UBE2D1 and COX7C as Potential Biomarkers of Diabetes-Related Sepsis. Biomed Res Int. 2022 Apr
Subcellular Location
Cytoplasm.
Synonyms
E2(17)KB1 antibody
SFT antibody
Stimulator of Fe transport antibody
UB2D1_HUMAN antibody
UBC 4/5 antibody
UBC4/5 antibody
UBC4/5 homolog antibody
UBC4/5, S. cerevisiae, homolog of antibody
UBCH 5 antibody
UBCH 5A antibody
ExpandImages
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Western blot analysis of SFT on different lysates with Rabbit anti-SFT antibody (HA722578) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: Raji cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse kidney tissue lysate
Lane 7: Rat kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 15 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722578) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
SFT was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722578 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722578 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate (input)
Lane 2: HA722578 IP in Jurkat cell lysate
Lane 3: Rabbit IgG instead of HA722578 in Jurkat cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 26 seconds; ECL: K1801 -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SFT antibody (HA722578) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722578) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma tissue with Rabbit anti-SFT antibody (HA722578) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722578) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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