SKP2 Recombinant Rabbit Monoclonal Antibody [PSH06-22] - BSA and Azide free
Catalog# HA751060
SKP2 Recombinant Rabbit Monoclonal Antibody [PSH06-22] - BSA and Azide free
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WB
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Human
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Green monkey
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unconjugated
Overview
Product Name
SKP2 Recombinant Rabbit Monoclonal Antibody [PSH06-22] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SKP2 aa 375-424.
Species Reactivity
Human, Green monkey
Validated Applications
WB
Target Molecular Weight
Predicted band size: 48 kDa
Positive Control
MDA-MB-231 cell lysate, 293T cell lysate, MDA-MB-468 cell lysate, HepG2 cell lysate, SK-OV-3 cell lysate, SH-SY5Y cell lysate, COS-1 cell lysate.
Conjugation
unconjugated
Clone Number
PSH06-22
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
S-phase kinase-associated protein 2 is an enzyme that in humans is encoded by the SKP2 gene. Skp2 contains 424 residues in total with the ~40 amino acid F-box domain lying closer to the N-terminal region at the 94-140 position and the C-terminal region forming a concave surface consisting of ten leucine-rich repeats (LRRs). The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which often—but not always—recognize substrates in a phosphorylation-dependent manner. In this SCF complex, Skp2 acts as the substrate recognition factor.
Background References
1. Wu T et al. Emerging Roles of SKP2 in Cancer Drug Resistance. Cells. 2021 May
2. Feng T et al. Skp2: A critical molecule for ubiquitination and its role in cancer. Life Sci. 2024 Feb
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
CDK2/Cyclin A associated protein p45 antibody
Cyclin A/CDK2 associated protein p45 antibody
Cyclin-A/CDK2-associated protein p45 antibody
F box protein Skp2 antibody
F box/LRR repeat protein 1 antibody
F-box protein Skp2 antibody
F-box/LRR-repeat protein 1 antibody
FBL 1 antibody
FBL1 antibody
FBXL 1 antibody
ExpandImages
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This data was developed using HA722654, the same antibody clone in a different buffer formulation.
Western blot analysis of SKP2 on different lysates with Rabbit anti-SKP2 antibody (HA722654) at 1/1,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: 293T cell lysate
Lane 3: MDA-MB-468 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: SK-OV-3 cell lysate
Lane 6: SH-SY5Y cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722654) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
SKP2 Recombinant Rabbit Monoclonal Antibody [PSH06-22]
Application: WB
Reactivity: Human,Green monkey
Conjugate: unconjugated
