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SLC7A5 / LAT1 Recombinant Rabbit Monoclonal Antibody [PSH09-28]

Usd: 385

Specification

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Catalog# HA723086

SLC7A5 / LAT1 Recombinant Rabbit Monoclonal Antibody [PSH09-28]

Application

  • WB

  • IF-Cell

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SLC7A5 / LAT1 Recombinant Rabbit Monoclonal Antibody [PSH09-28]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SLC7A5 aa 71-507.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, IP

Molecular Weight

Predicted band size: 55 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, HT-29, human testis tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

PSH09-28

Reactivity Data

WB IF-Cell IHC-P IP
Human
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested Tested
Not Recommended
Rat
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:200

  • IP

  • 1-2μg/sample

Target

Function

Large neutral amino acids transporter small subunit 1, also known as 4F2 light chain, or CD98 light chain is a protein that in humans is encoded by the SLC7A5 gene. Enables L-leucine transmembrane transporter activity; L-tryptophan transmembrane transporter activity; and thyroid hormone transmembrane transporter activity. Involved in carboxylic acid transport; thyroid hormone transport; and xenobiotic transport. Located in cytosol; intracellular membrane-bounded organelle; and plasma membrane. Is integral component of membrane. Part of amino acid transport complex; apical plasma membrane; and microvillus membrane.

Background References

1. Najumudeen AK et al. The amino acid transporter SLC7A5 is required for efficient growth of KRAS-mutant colorectal cancer. Nat Genet. 2021 Jan

2. Nachef M et al. Targeting SLC1A5 and SLC3A2/SLC7A5 as a Potential Strategy to Strengthen Anti-Tumor Immunity in the Tumor Microenvironment. Front Immunol. 2021 Apr

Subcellular Location

Apical cell membrane, Cell membrane, Lysosome membrane.

UNIPROT

SwissProt: Q01650 Human

SwissProt: Q9Z127 Mouse

SwissProt: Q63016 Rat

Synonyms

4F2 LC antibody

4F2 light chain antibody

4F2LC antibody

CD98 antibody

CD98 light chain antibody

CD98LC antibody

D16S469E antibody

DC49 antibody

E16 antibody

hLAT1 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of SLC7A5 / LAT1 on different lysates with Rabbit anti-SLC7A5 / LAT1 antibody (<a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: LNCaP cell lysate (negative) (20 µg/Lane)<br />Lane 3: Jurkat cell lysate (20 µg/Lane)<br />Lane 4: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 5: Rat testis tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 55 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: Lane 1-2: 1 minute; Lane 3-5: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of SLC7A5 / LAT1 on different lysates with Rabbit anti-SLC7A5 / LAT1 antibody (HA723086) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: LNCaP cell lysate (negative) (20 µg/Lane)
    Lane 3: Jurkat cell lysate (20 µg/Lane)
    Lane 4: Mouse testis tissue lysate (40 µg/Lane)
    Lane 5: Rat testis tissue lysate (40 µg/Lane)

    Predicted band size: 55 kDa
    Observed band size: 37 kDa

    Exposure time: Lane 1-2: 1 minute; Lane 3-5: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723086) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunocytochemistry analysis of HT-29 (positive) and LNCaP (negative) labeling SLC7A5 / LAT1 with Rabbit anti-SLC7A5 / LAT1 antibody (<a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLC7A5 / LAT1 antibody (<a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    ☑ Relative expression (RE)

    Immunocytochemistry analysis of HT-29 (positive) and LNCaP (negative) labeling SLC7A5 / LAT1 with Rabbit anti-SLC7A5 / LAT1 antibody (HA723086) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLC7A5 / LAT1 antibody (HA723086) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SLC7A5 / LAT1 antibody (<a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SLC7A5 / LAT1 antibody (HA723086) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723086) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SLC7A5 / LAT1 antibody (<a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SLC7A5 / LAT1 antibody (HA723086) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA723086) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SLC7A5 / LAT1 was immunoprecipitated from 0.2 mg A549 cell lysate with <a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a> at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (<a href="/products/M1208-2" style="font-weight: bold;text-decoration: underline;">M1208-2</a>) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: A549 cell lysate (input)<br />Lane 2: <a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a> IP in A549 cell lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA723086" style="font-weight: bold;text-decoration: underline;">HA723086</a> in A549 cell lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 59 seconds; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    SLC7A5 / LAT1 was immunoprecipitated from 0.2 mg A549 cell lysate with HA723086 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723086 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: A549 cell lysate (input)
    Lane 2: HA723086 IP in A549 cell lysate
    Lane 3: Rabbit IgG instead of HA723086 in A549 cell lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 59 seconds; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

SLC7A5 / LAT1 Recombinant Rabbit Monoclonal Antibody [PSH09-28] - BSA and Azide free

Application: WB,IF-Cell,IHC-P,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated