SMURF1 Recombinant Rabbit Monoclonal Antibody [PSH10-12] - BSA and Azide free
Catalog# HA751341
SMURF1 Recombinant Rabbit Monoclonal Antibody [PSH10-12] - BSA and Azide free
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WB
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Human
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unconjugated
Overview
Product Name
SMURF1 Recombinant Rabbit Monoclonal Antibody [PSH10-12] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human SMURF1 aa
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 86 kDa
Positive Control
HeLa cell lysate, MCF7 cell lysate, 293T cell lysate, 786-0 cell lysate, LNCaP cell lysate, U-2 OS cell lysate, A549 cell lysate.
Conjugation
unconjugated
Clone Number
PSH10-12
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
Target
Function
E3 ubiquitin-protein ligase SMURF1 is an enzyme that in humans is encoded by the SMURF1 gene. The SMURF1 Gene encodes a protein with a size of 757 amino acids and the molecular mass of this protein is 86114 Da. Smad ubiquitination regulatory factor 1 (Smurf1) is part of a gene that encodes a ubiquitin ligase and is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined.
Background References
1. Xia Q et al. SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis. Autophagy. 2024 Apr
2. Peng Z et al. Targeting Smurf1 to block PDK1-Akt signaling in KRAS-mutated colorectal cancer. Nat Chem Biol. 2024 Jul
Subcellular Location
Cytoplasm, Cell membrane.
UNIPROT
Synonyms
E3 ubiquitin-protein ligase SMURF1 antibody
hSMURF1 antibody
KIAA1625 antibody
Smad specific E3 ubiquitin ligase 1 antibody
SMAD specific E3 ubiquitin protein ligase 1 antibody
Smad ubiquitination regulatory factor 1 antibody
SMAD-specific E3 ubiquitin-protein ligase 1 antibody
SMUF1_HUMAN antibody
SMURF 1 antibody
smurf1 antibody
Images
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This data was developed using HA723181, the same antibody clone in a different buffer formulation.
Western blot analysis of SMURF1 on different lysates with Rabbit anti-SMURF1 antibody (HA723181) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: 293T cell lysate
Lane 4: 786-0 cell lysate
Lane 5: LNCaP cell lysate
Lane 6: U-2 OS cell lysate
Lane 7: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 87 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723181) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
SMURF1 Rabbit Polyclonal Antibody
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
