SOX2 Mouse Monoclonal Antibody [C9-F10]
Catalog# EM1708-84
SOX2 Mouse Monoclonal Antibody [C9-F10]
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
SOX2 Mouse Monoclonal Antibody [C9-F10]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
34 kDa
Positive Control
SOX2-hIgGFc transfected HEK293 cell lysate, human lung cancer tissue, human esophageal cancer tissue, NTERA-2.
Conjugation
unconjugated
Clone Number
C9-F10
Product Features
Form
Liquid
Concentration
Storage Instructions
4℃; -20℃ for long term storage.
Storage Buffer
Purified antibody in PBS with 0.05% sodium azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IF-cell
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1:50-1:200
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IHC-P
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1:100-1:500
Target
Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities.
Background References
1. Li Y et al. Abnormal Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Partially Mimicked Development of TSC2 Neurological Abnormalities. Stem Cell Reports 8:883-893 (2017).
2. Yuan J et al. M2 microglia promotes neurogenesis and oligodendrogenesis from neural stem/progenitor cells via the PPAR signaling pathway. Oncotarget 8:19855-19865 (2017).
Subcellular Location
Nucleus.
UNIPROT
Synonyms
ANOP3 antibody
cb236 antibody
Delta EF2a antibody
lcc antibody
MCOPS3 antibody
MGC148683 antibody
MGC2413 antibody
RGD1565646 antibody
Sex determining region Y box 2 antibody
SOX 2 antibody
ExpandImages
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Western blot analysis of SOX2 on HEK293 (1) and SOX2-hIgGFc transfected HEK293 (2) cell lysate using anti-SOX2 antibody at 1/1,000 dilution.
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ICC staining SOX2 (green) and Actin filaments (red) in NTERA-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-SOX2 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-SOX2 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
SOX2 Recombinant Rabbit Monoclonal Antibody [PO00-28]
Application: IHC-P,WB,IF-Cell,ChIP,IHC-Fr,IF-Tissue,mIHC
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
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