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SQSTM1 / p62 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# R1309-8

SQSTM1 / p62 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

SQSTM1 / p62 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human TESPA1 aa 27-91.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

62kDa

Positive Control

Hela, F9, HepG2, human ovary carcinoma tissue

Conjugation

unconjugated

RRID

AB_3073248

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:200

Target

Function

Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. It may play a role in titin/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. P62 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

Background References

1. "p62 overexpression in breast tumors and regulation by prostate-derived Ets factor in breast cancer cells." Thompson H.G.R., Harris J.W., Wold B.J., Lin F., Brody J.P. Oncogene 22:2322-2333(2003)

2. "Ubiquitin recognition by the ubiquitin-associated domain of p62 involves a novel conformational switch." Long J., Gallagher T.R., Cavey J.R., Sheppard P.W., Ralston S.H., Layfield R., Searle M.S. J. Biol. Chem. 283:5427-5440(2008)

Tissue Specificity

Ubiquitously expressed.

Post-translational Modification

Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN. Phosphorylation at Ser-403 by ULK1 is stimulated by SESN2. Phosphorylated at Ser-403 by TBK1, leading to promote relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Phosphorylation at Ser-349 by MTOR promotes interaction with KEAP1 and inactivation of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes (By similarity).; Ubiquitinated by RNF26: ubiquitinated SQSTM1 attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Deubiquitination by USP15 releases target vesicles for fast transport into the cell periphery. Ubiquitinated by the BCR(KEAP1) complex at Lys-420, increasing SQSTM1 sequestering activity and promoting its degradation. Ubiquitinated via 'Lys-29' and 'Lys-33'-linked polyubiquitination leading to xenophagic targeting of bacteria and inhibition of their replication.

Subcellular Location

Cytoplasm

UNIPROT

SwissProt: Q13501 Human

Synonyms

A170 antibody

DMRV antibody

EBI 3 associated protein of 60 kDa antibody

EBI 3 associated protein p60 antibody

EBI3 associated protein of 60 kDa antibody

EBI3 associated protein p60 antibody

EBI3-associated protein of 60 kDa antibody

EBIAP antibody

FTDALS3 antibody

MGC127197 antibody

Expand

Images

  • Western blot analysis on Hela (1) and F9 (2) cell lysates using anti-SQSTM1 rabbit polyclonal antibodies.

    Western blot analysis on Hela (1) and F9 (2) cell lysates using anti-SQSTM1 rabbit polyclonal antibodies.

  • ICC staining SQSTM1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

    ICC staining SQSTM1 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

  • Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue using anti- SQSTM1 rabbit polyclonal antibody.

    Immunohistochemical analysis of paraffin-embedded human ovary carcinoma tissue using anti- SQSTM1 rabbit polyclonal antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Alternative Products

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SQSTM1 / p62 Recombinant Rabbit Monoclonal Antibody [PS00-61]

Application: WB,IHC-P,IF-Cell,FC,IF-Tissue,IHC-Fr

Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig

Conjugate: unconjugated

Products with the same target and pathway

metafield image

SQSTM1 / p62 Mouse Monoclonal Antibody [10-E10]

Application: IF-Cell,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated