ST2 Mouse Monoclonal Antibody [PSH01-68]
Catalog# HA601170
ST2 Mouse Monoclonal Antibody [PSH01-68]
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WB
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IF-Cell
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FC
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Human
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unconjugated
Overview
Product Name
ST2 Mouse Monoclonal Antibody [PSH01-68]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human ST2 aa 19-328 (Extracellular).
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 63.4 kDa
Positive Control
HeLa transfected with FLAG-tagged ST2 cell lysate, HeLa overexpress with ST2.
Conjugation
unconjugated
Clone Number
PSH01-68
RRID
Reactivity Data
| WB | IF-Cell | FC | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
||
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
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FC
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1:1,000
Target
Function
Receptor for interleukin-33 (IL-33); signaling requires association of the coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages.
Background References
1. Schmitz J., Owyang A., Oldham E., Song Y., Murphy E., McClanahan T.K., Zurawski G., Moshrefi M., Qin J., Li X., Gorman D.M., Bazan J.F., Kastelein R.A. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor- related protein ST 2 and induces T helper type 2-associated cytokines. Immunity 23:479-490 (2005)
Subcellular Location
Cell membrane, Membrane, Secreted.
UNIPROT
Synonyms
DER-4 antibody
DER4 antibody
FIT 1 antibody
Growth stimulation expressed antibody
homolog of mouse growth stimulation-expressed antibody
Il1rl1 antibody
IL33R antibody
ILRL1_HUMAN antibody
Interleukin 1 receptor like 1 antibody
interleukin 1 receptor related protein antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of ST2 on different lysates with Mouse anti-ST2 antibody (HA601170) at 1/1,000 dilution.
Lane 1: HeLa transfected with FLAG-tagged empty control cell lysate
Lane 2: HeLa transfected with FLAG-tagged ST2 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 63 kDa
Observed band size: 80 kDa
Exposure time: 5 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601170) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Immunocytochemistry analysis of HeLa overexpress with or without ST2 cells labeling ST2 with Mouse anti-ST2 antibody (HA601170) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ST2 antibody (HA601170) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
☑ Cell treatment (CT)
Flow cytometric analysis of HeLa overexpress with or without ST2 cells labeling ST2.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601170, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1126) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
ST2 Mouse Monoclonal Antibody [PSH01-69]
Application: WB,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
