STAT6 Recombinant Rabbit Monoclonal Antibody [SY13-09]
Catalog# ET1605-49
STAT6 Recombinant Rabbit Monoclonal Antibody [SY13-09]
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WB
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IF-Cell
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IHC-P
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IP
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FC
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IF-Tissue
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
STAT6 Recombinant Rabbit Monoclonal Antibody [SY13-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human STAT6 C terminal.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, IP, FC, IF-Tissue
Molecular Weight
Predicted band size: 94 kDa
Positive Control
Hela-si NT cell lysate, Hela-si STAT6 cell lysate, THP-1 cell lysate, Jurkat cell lysate, Raji cell lysate, HeLa cell lysate, MDA-MB-231 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, human solitary fibrous tumor tissue, Hela, HepG2, NIH/3T3.
Conjugation
unconjugated
Clone Number
SY13-09
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:200-1:500
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IHC-P
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1:3,000
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IF-Tissue
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1:1,000
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
Target
Function
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of proteins that have been designated Stats (signal transducers and activators of transcription). The first members of this family to be described include Stat1α p91, Stat1β p84 (a form of p91 that lacks 38 COOH-terminal amino acids) and Stat2 p113. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Stat3, which becomes activated in response to epidermal growth factor (EGF) and interleukin-6 (IL-6), but not interferon-γ (IFN-γ) or Stat4, is an additional member of this family. It has been suggested that the phosphorylated forms of both Stat3 and Stat4 form homodimers as well as heterodimers with the other members of the Stat family, and that differential activation of different Stat proteins in response to different ligands should help to explain specificity in nuclear signaling from the cell surface. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Other members of the Stat family include Stat5, which has been shown to be activated by prolactin and by IL-3, and Stat6 (also designated IL-4 Stat), which is involved in IL-4-activated signaling pathways.
Background References
1. Zheng, C. et al. 2015. CD11b regulates obesity-induced insulin resistance via limiting alternative activation and proliferation of adipose tissue macrophages. Proc. Natl. Acad. Sci. U.S.A. 112: E7239-48.
2. Carlson, TJ. et al. 2014. Halofuginone-induced amino acid starvation regulates Stat3-dependent Th17 effector function and reduces established autoimmune inflammation. J. Immunol. 192: 2167-76.
Sequence Similarity
Belongs to the transcription factor STAT family.
Post-translational Modification
Tyrosine phosphorylated on Tyr-641 following stimulation by IL4/interleukin-4. Tyrosine phosphorylated following stimulation by IL3/interleukin-3 (By similarity). Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling.; Mono-ADP-ribosylated by PARP14.
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
12S1644 antibody
D12S1644 antibody
IL 4 STAT antibody
IL-4 Stat antibody
IL4 STAT antibody
Interleukin 4 Induced antibody
Interleukin 4 Induced Transcription Factor IL4 STAT antibody
Signal transducer and activator of transcription 6 antibody
Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
ExpandImages
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Western blot analysis of STAT6 on different lysates with Rabbit anti-STAT6 antibody (ET1605-49) at 1/2,000 dilution.
Lane 1: THP-1 cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: Raji cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: RAW264.7 cell lysate (20 µg/Lane)
Predicted band size: 94 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-49) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of STAT6 on different lysates with Rabbit anti-STAT6 antibody (ET1605-49) at 1/500 dilution.
Lane 1: Hela-si NT cell lysate
Lane 2: Hela-si STAT6 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 94 kDa
Observed band size: 100 kDa
Exposure time: 1 minute 34 seconds;
4-20% SDS-PAGE gel.
ET1605-49 was shown to specifically react with STAT6 in Hela-si NT cells. Weakened band was observed when Hela-si STAT6 sample was tested. Hela-si NT and Hela-si STAT6 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1605-49, 1/500) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
STAT6 was immunoprecipitated from 0.5 mg Hela whole cell lysates with ET1605-49 at 2 μg/mL. Western blot was performed from the immunoprecipitate using ET1605-49 at 1/500 dilution for 45 minutes at room temperature. Goat anti-Rabbit IgG-HRP Secondary Antibody (HA1001) was used at 1:300,000 dilution for 30 minutes at room temperature.
Lane 1: Hela whole cell lysates at 10 μg;
Lane 2: STAT6 (ET1605-49) IP in Hela whole cell lysates;
Lane 3: Rabbit IgG instead of STAT6 (ET1605-49) in Hela whole cell lysates.
Predicted band size: 94 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel. -
Immunohistochemical analysis of paraffin-embedded human solitary fibrous tumor tissue with Rabbit anti-STAT6 antibody (ET1605-49) at 1/1,000 dilution.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with ET1605-49 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-STAT6 antibody (ET1605-49) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-49) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-STAT6 antibody (ET1605-49) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-49) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-STAT6 antibody (ET1605-49) at 1/3,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-49) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of MDA-MB-231 cells labeling STAT6 with Rabbit anti-STAT6 antibody (ET1605-49) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-STAT6 antibody (ET1605-49) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HeLa cells labeling STAT6.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1605-49, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Application: IF-Tissue
Species: Mouse
Site: kidney
Sample: Paraffin-embedded section
Antibody concentration: 1/1,000 -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Solitary fibrous tumor
Sample: Paraffin-embedded section
Primary antibody dilution: 1/1,000
Antigen retrieval: ER2
Platform: Leica Biosystems BOND® RX
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
-
Astragali radix - Curcumae rhizoma herb pair enhances Sorafenib's efficacy by inducing ferroptosis and activates Th1 cell immune response synergistically against hepatocellular carcinoma
Journal: Phytomedicine
DOI: 10.1016/j.phymed.2025.157326
IF: 8.3
Application: IHC
Reactivity: Mouse
Publish date: 2025 Sept
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Self-assembled PROTACs enable protein degradation to reprogram the tumor microenvironment for synergistically enhanced colorectal cancer immunotherapy
Journal: Bioactive Materials
DOI:
IF: 18
Application: WB
Reactivity: Mouse
Publish date: 2024 Oct
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