Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH06-58]
Catalog# HA722721
Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH06-58]
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WB
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IHC-P
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Human
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Mouse
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Rat
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HA751086
不含抗保成分
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unconjugated
Overview
Product Name
Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH06-58]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human MYH7 aa 1,250-1,300.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 223 kDa
Positive Control
Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, human skeletal muscle tissue.
Conjugation
unconjugated
Clone Number
PSH06-58
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:2,000
Target
Function
MYH7 is a gene encoding a myosin heavy chain beta (MHC-β) isoform (slow twitch) expressed primarily in the heart, but also in skeletal muscles (type I fibers). This isoform is distinct from the fast isoform of cardiac myosin heavy chain, MYH6, referred to as MHC-α. MHC-β is the major protein comprising the thick filament that forms the sarcomeres in cardiac muscle and plays a major role in cardiac muscle contraction.
Background References
1. Gao Y et al. MYH7 in cardiomyopathy and skeletal muscle myopathy. Mol Cell Biochem. 2024 Feb
2. Park J et al. A genome-first approach to rare variants in hypertrophic cardiomyopathy genes MYBPC3 and MYH7 in a medical biobank. Hum Mol Genet. 2022 Mar
Subcellular Location
Cytoplasm, myofibril, sarcomere.
Synonyms
Beta myosin heavy chain antibody
cardiac muscle beta isoform antibody
CMD1S antibody
CMH1 antibody
MPD1 antibody
MYH7 antibody
MYH7_HUMAN antibody
Myhc slow antibody
MyHC-beta antibody
MyHC-slow antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Slow Skeletal Myosin Heavy chain on different lysates with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722721) at 1/1,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate (40 µg/Lane)
Lane 2: Mouse spleen tissue lysate (negative) (40 µg/Lane)
Lane 3: Rat skeletal muscle tissue lysate (40 µg/Lane)
Lane 4: Rat spleen tissue lysate (negative) (40 µg/Lane)
Predicted band size: 223 kDa
Observed band size: 223 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722721) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722721) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722721) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human spleen tissue (negative) with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody (HA722721) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722721) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH07-54] - BSA and Azide free
Application: WB,IHC-P,IF-Tissue,IHC-Fr
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody [PSH07-54]
Application: WB,IHC-P,IF-Tissue,IHC-Fr,mIHC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
