Skip to content

Smad1/5/9 Rabbit Polyclonal Antibody

Usd:

Bulk Order Quote | Customization Request

Catalog# ER64980

Smad1/5/9 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Smad1/5/9 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

The antiserum was produced against synthesized peptide derived from human Smad1/5/9. AA range:10-59

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

56 kDa

Positive Control

Kidney,Mammary carcinoma,Placenta,Uterus,

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2000

  • IHC-P

  • 1:100-1:300

Target

Function

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-med

Background References

暂无

UNIPROT

SwissProt: Q15797 Human

SwissProt: Q99717 Human

SwissProt: O15198 Human

Synonyms

BSP-1 antibody

BSP1 antibody

HsMAD1 antibody

JV4-1 antibody

JV41 antibody

MAD homolog 1 antibody

MAD mothers against decapentaplegic homolog 1 antibody

Mad related protein 1 antibody

Mad-related protein 1 antibody

MADH1 antibody

Expand

Images

  • Western Blot analysis of Hela cell lysate using Smad1/5/9 Polyclonal Antibody diluted at 1:500

    Western Blot analysis of Hela cell lysate using Smad1/5/9 Polyclonal Antibody diluted at 1:500

  • Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

    Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

Phospho-Smad1/5/9 (S463/S465/S467) Recombinant Rabbit Monoclonal Antibody [JE59-46]

Application: WB,IHC-P,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated